ArticleStem cell reports2020
Inhibition of Apoptosis Reduces Diploidization of Haploid Mouse Embryonic Stem Cells during Differentiation.
Article in Stem cell reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Metabolic Imbalance Triggers Adaptive Remodeling to Accelerate Diploidization in Murine Haploid Embryonic Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Mitochondrial metabolic imbalance drives diploidization in mouse haploid embryonic stem cells via NADPH overload.Nature communications · 2026Article
- Genetic Screening of Haploid Neural Stem Cells Reveals that Nfkbia and Atp2b4 are Key Regulators of Oxidative Stress in Neural Precursors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Development and application of haploid embryonic stem cells.Stem cell research & therapy · 2024Review
- Haploid-genetic screening of trophectoderm specification identifiesScience advances · 2023Article
- BCL2 is a major regulator of haploidy maintenance in murine embryonic stem cells.Cell proliferation · 2023Article
- Transcriptome profiles and chromatin states in mouse androgenetic haploid embryonic stem cells.Cell proliferation · 2023Article
- Generation of a human haploid neural stem cell line for genome-wide genetic screening.World journal of stem cells · 2023Article
- Visual evoked potentials waveform analysis to measure intracortical damage in a preclinical model of multiple sclerosis.Frontiers in cellular neuroscience · 2023Article
- miR-30e-5p attenuates neuronal deficit and inflammation of rats with intracerebral hemorrhage by regulating TLR4.Experimental and therapeutic medicine · 2022Article
- Genome-scale screening in a rat haploid system identifies Thop1 as a modulator of pluripotency exit.Cell proliferation · 2022Article
- Recombinant human erythropoietin attenuates hepatic dysfunction by suppressing hepatocellular apoptosis in lipopolysaccharide-induced disseminated intravascular coagulation in rats.Biomedical reports · 2022Article
- Genetically engineered pigs for xenotransplantation: Hopes and challenges.Frontiers in cell and developmental biology · 2022Review
- PINCH1 knockout aggravates myocardial infarction in mice via mediating the NF-κB signaling pathway.Experimental and therapeutic medicine · 2022Article
- Dipeptidyl peptidase-4 inhibition prevents lung injury in mice under chronic stress via the modulation of oxidative stress and inflammation.Experimental animals · 2021Article
- High-throughput screening in postimplantation haploid epiblast stem cells reveals Hs3st3b1 as a modulator for reprogramming.Stem cells translational medicine · 2021Article
- A Genetic Screen Identifies Etl4-Deficiency Capable of Stabilizing the Haploidy in Embryonic Stem Cells.Stem cell reports · 2021Article
- The milestone of genetic screening: Mammalian haploid cells.Computational and structural biotechnology journal · 2020Review
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phenotypes of haploid embryonic stem cells (haESCs) are dominant for recessive traits in mice. However, one major obstacle to their use is self-diploidization in daily culture. Although haESCs maintain haploidy well by deleting p53, whether they can sustain haploidy in differentiated status and the mechanism behind it remain unknown. To address this, we induced p53-deficient haESCs into multiple differentiated lineages maintain haploid status in vitro. Haploid cells also remained in chimeric embryos and teratomas arising from p53-null haESCs. Transcriptome analysis revealed that apoptosis genes were downregulated in p53-null haESCs compared with that in wild-type haESCs. Finally, we knocked out p73, another apoptosis-related gene, and observed stabilization of haploidy in haESCs. These results indicated that the main mechanism of diploidization was apoptosis-related gene-triggered cell death in haploid cell cultures. Thus, we can derive haploid somatic cells by manipulating the apoptosis gene, facilitating genetic screens of lineage-specific development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.