Evidence map›Paper›PMID 32502348›Full record

ArticleThe Journal of organic chemistry2020

Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis.

Thomas M Haas, Danye Qiu, Markus Häner, Larissa Angebauer, Alexander Ripp, Jyoti Singh, Hans-Georg Koch, Claudia Jessen-Trefzer, Henning J Jessen

Open access · bronzeAbstract read
In one paragraph

Article in The Journal of organic chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Bacterial Pathogen Infection Triggers Magic Spot Nucleotide Signaling inJournal of the American Chemical Society · 2023
    Article
  7. Photoaffinity Capture Compounds to Profile the Magic Spot Nucleotide Interactomes.Angewandte Chemie (International ed. in English) · 2022
    Article
  8. Review
  9. Review
  10. Article
  11. The Dynamic SecYEG Translocon.Frontiers in molecular biosciences · 2021
    Review
  12. Estimates of RelFrontiers in microbiology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Thomas M HaasInstitute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
Danye QiuInstitute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
Markus HänerInstitute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
Larissa AngebauerInstitute of Biochemistry and Molecular Biology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.
Alexander RippInstitute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
Jyoti SinghInstitute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
Hans-Georg KochInstitute of Biochemistry and Molecular Biology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.ORCID 0000-0001-5913-0334
Claudia Jessen-TrefzerInstitute of Pharmaceutical Biology and Biotechnology, University of Freiburg, 79104 Freiburg, Germany.ORCID 0000-0003-4216-8189
Henning J JessenInstitute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.ORCID 0000-0002-1025-9484
University of Freiburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.

Identifiers

PMID32502348
PMCPMC7684580
OpenAlexW3033326246

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.