ArticleNucleic acids research2020
Argonaute binding within 3'-untranslated regions poorly predicts gene repression.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 52 citations in OpenAlex.
- StringTie3 improves total RNA-seq assembly by resolving nascent and mature transcripts.Nature methods · 2026Article
- 3'-tRNA Fragments Target Domesticated LTR-Retrotransposons.bioRxiv : the preprint server for biology · 2026Article
- Nuclear Argonaute:miRNA complexes recognize target sequences within chromatin-associated RNA and silence gene expression.Nucleic acids research · 2025Article
- g.nome, A Transparent Bioinformatics Pipeline that Enables Differential Expression and Alternative Splicing Analysis by Non-Computational Biologists.bioRxiv : the preprint server for biology · 2025Article
- Mapping effective microRNA pairing beyond the seed using abasic modifications.Nucleic acids research · 2025Article
- It is Time to Revisit miRNA Therapeutics.Nucleic acid therapeutics · 2025Review
- Target cleavage and gene silencing by Argonautes with cityRNAs.Cell reports · 2024Article
- The Biosynthesis Process of Small RNA and Its Pivotal Roles in Plant Development.International journal of molecular sciences · 2024Review
- RNA-guided RNA silencing by an Asgard archaeal Argonaute.Nature communications · 2024Article
- RNA-RNA interactions between respiratory syncytial virus and miR-26 and miR-27 are associated with regulation of cell cycle and antiviral immunity.Nucleic acids research · 2024Article
- Experimental capture of miRNA targetomes: disease-specific 3'UTR library-based miRNA targetomics for Parkinson's disease.Experimental & molecular medicine · 2024Article
- The miRNA-target interactions: An underestimated intricacy.Nucleic acids research · 2024Review
- Nuclear localization of Argonaute 2 is affected by cell density and may relieve repression by microRNAs.Nucleic acids research · 2024Article
- Skin treatment with non-thermal plasma modulates the immune system through miR-223-3p and its target genes.RNA biology · 2024Article
- The Mechanisms of miRNAs on Target Regulation and their Recent Advances in Atherosclerosis.Current medicinal chemistry · 2024Review
- On the rules of engagement for microRNAs targeting protein coding regions.Nucleic acids research · 2023Article
- Insights in piRNA targeting rules.Wiley interdisciplinary reviews. RNA · 2023Article
- Consequences of depleting TNRC6, AGO, and DROSHA proteins on expression of microRNAs.RNA (New York, N.Y.) · 2023Article
- Analysis of the Contribution of 6-mer Seed Toxicity to HIV-1-Induced Cytopathicity.Journal of virology · 2023Article
- Nuclear Localization of Argonaute is affected by Cell Density and May Relieve Repression by microRNAs.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Despite two decades of study, the full scope of RNAi in mammalian cells has remained obscure. Here we combine: (i) Knockout of argonaute (AGO) variants; (ii) RNA sequencing analysis of gene expression changes and (iii) Enhanced Crosslinking Immunoprecipitation Sequencing (eCLIP-seq) using anti-AGO2 antibody to identify potential microRNA (miRNA) binding sites. We find that knocking out AGO1, AGO2 and AGO3 together are necessary to achieve full impact on steady state levels of mRNA. eCLIP-seq located AGO2 protein associations within 3'-untranslated regions. The standard mechanism of miRNA action would suggest that these associations should repress gene expression. Contrary to this expectation, associations between AGO and RNA are poorly correlated with gene repression in wild-type versus knockout cells. Many clusters are associated with increased steady state levels of mRNA in wild-type versus knock out cells, including the strongest cluster within the MYC 3'-UTR. Our results suggest that assumptions about miRNA action should be re-examined.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.