Evidence map›Paper›PMID 32500713›Full record

ArticleJournal of proteome research2020

Optimal Dissociation Methods Differ for

Nicholas M Riley, Stacy A Malaker, Marc D Driessen, Carolyn R Bertozzi

Abstract read
In one paragraph

Article in Journal of proteome research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 129 papers.

0numbers the graph read from it
0cells of the map it votes in
129citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

129 citing papers in PubMed.

  1. Site-specific O-glycans influence lacritin structure and multimerization in tears.Protein science : a publication of the Protein Society · 2026
    Article
  2. Article
  3. Deep Learning Prediction of O-Glycopeptide Tandem Mass Spectra Enhances O-Glycoproteomics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Structural and functional characterization of human kallistatin.Biochemistry and biophysics reports · 2026
    Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. The protease cathepsin K can debulk the cancer glycocalyx.The Journal of biological chemistry · 2026
    Article
  15. Article
  16. Article
  17. PeerJ · 2026
    Review
  18. Article
  19. Article
  20. Article

69 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Nicholas M RileyDepartment of Chemistry, Stanford University, Stanford, California 94305-6104, United States.ORCID 0000-0002-1536-2966
Stacy A MalakerDepartment of Chemistry, Stanford University, Stanford, California 94305-6104, United States.ORCID 0000-0003-2382-5067
Marc D DriessenDepartment of Chemistry, Stanford University, Stanford, California 94305-6104, United States.
Carolyn R BertozziDepartment of Chemistry, Stanford University, Stanford, California 94305-6104, United States.ORCID 0000-0003-4482-2754

Funding

Investigating the Surface GlycoproteomeR01CA200423 · NCI · STANFORD UNIVERSITY · PI Carolyn Bertozzi · 2015 to 2026
$3.8M
Making glycoproteomics via mass spectrometry more accessible to the greater scientific communityU01CA207702 · NCI · STANFORD UNIVERSITY · PI BERTOZZI, CAROLYN, PITTERI, SHARON · 2016 to 2019
$1.9M
Uniting Mass Spectrometry and Glycoscience to Investigate Cancer BiologyK00CA212454 · NCI · STANFORD UNIVERSITY · PI RILEY, NICHOLAS M · 2018 to 2021
$331k
An enzymatic approach to study cancer-associated cell-surface glycoproteins: exploration of mucin-degrading bacterial metalloproteasesF32GM126663 · NIGMS · STANFORD UNIVERSITY · PI MALAKER, STACY ALYSE · 2017 to 2019
$125k
Howard Hughes Medical InstituteNCI NIH HHS K00 CA212454NCI NIH HHS R01 CA200423NCI NIH HHS U01 CA207702NIGMS NIH HHS F32 GM126663
6 · The paper itself

Abstract

Site-specific characterization of glycosylation requires intact glycopeptide analysis, and recent efforts have focused on how to best interrogate glycopeptides using tandem mass spectrometry (MS/MS). Beam-type collisional activation, i.e., higher-energy collisional dissociation (HCD), has been a valuable approach, but stepped collision energy HCD (sceHCD) and electron transfer dissociation with HCD supplemental activation (EThcD) have emerged as potentially more suitable alternatives. Both sceHCD and EThcD have been used with success in large-scale glycoproteomic experiments, but they each incur some degree of compromise. Most progress has occurred in the area of

Indexed as

GlycopeptidesTandem Mass SpectrometryGlycosylationGlycopeptideselectron transfer dissociationETDEThcDfragmentationglycoproteomicsN-glycopeptidesO-glycopeptidessceHCDstepped collision energy high-energy collisional dissociationtandem MS

Identifiers

PMID32500713
PMCPMC7425838

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.