Evidence map›Paper›PMID 32500507›Full record

ArticleActa parasitologica2020

Immunolocalization of Disorganized Muscle Protein-1 in Different Life Stages of Human Lymphatic Filariid, Brugia malayi.

Vikas Kushwaha, Amogh A Sahasrabuddhe, P Kalpana Murthy

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta parasitologica, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.0field-weighted citation impact, top 61% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Vikas KushwahaDepartment of Zoology, Faculty of Science, University of Lucknow, University Road, Lucknow, 226007, India.
Amogh A SahasrabuddheDivision of Molecular and Structural Biology, CSIR-Central Drug Research Institute, Lucknow, 226001, India.
P Kalpana MurthyDepartment of Zoology, Faculty of Science, University of Lucknow, University Road, Lucknow, 226007, India. drpkmurthy@yahoo.com.ORCID http://orcid.org/0000-0002-3499-2621
University of Lucknow · INCentral Drug Research Institute · IN

Funding

Council of Scientific and Industrial Research (IN) Emeritus Scientist Project (21(0963)/13/EMR-II)
6 · The paper itself

Abstract

purposeWe recently identified disorganized muscle protein-1 of Brugia malayi (DIM-1bm) as a vaccine candidate for human lymphatic filariasis. The present study was aimed at investigating the localization of DIM-1bm in the life-stages of B. malayi to identify the tissue target of vaccine action.

methodsRecombinant DIM-1bm (rDIM-1bm) was prepared and antibodies were raised in BALB/c mice. Immunoblots of SDS-PAGE resolved B. malayi infective 3rd stage larvae (L

resultsImmunofluorescence staining showed that DIM-1bm is localized mainly in the subcuticular muscle layer in the L

conclusionThe localization of DIM-1bm in the parasites' muscle layer suggests that the immunoprophylactic efficacy of DIM-1 is evidently due to immobilization of the parasite and its subsequent immune elimination.

Indexed as

Brugia malayiElephantiasis, FilarialAnimalsAntibodies, HelminthAntigens, HelminthFemaleHumansMiceMice, Inbred BALB CMuscle ProteinsAntibodies, HelminthAntigens, HelminthMuscle ProteinsBrugia malayiConfocal microscopyDisorganized muscle protein-1ImmunoblottingImmunolocalization

Identifiers

PMID32500507
PMCPMC7271955
OpenAlexW3033130579

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.