Evidence map›Paper›PMID 32500473›Full record

ArticleBiotechnology letters2020

Formaldehyde effects on kanamycin resistance gene of inactivated recombinant Escherichia coli vaccines.

Rafael A Donassolo, Marcos Roberto A Ferreira, Clóvis Moreira, Lucas M Dos Santos, Emili Griep, Gustavo M S G Moreira, Rafael R Rodrigues, Ângela N Moreira, Fabricio R Conceição

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In one paragraph

Article in Biotechnology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Rafael A DonassoloCentro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil. rafaeldonassolo@hotmail.com.ORCID http://orcid.org/0000-0002-9919-6845
Marcos Roberto A FerreiraCentro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil.
Clóvis MoreiraCentro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil.
Lucas M Dos SantosCentro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil.
Emili GriepCentro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil.
Gustavo M S G MoreiraInstitut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie , Spielmannstr, Technische Universität Braunschweig, Braunschweig, Germany.
Rafael R RodriguesInstitut für Biochemie, Biotechnologie und Bioinformatik, Abteilung Biotechnologie , Spielmannstr, Technische Universität Braunschweig, Braunschweig, Germany.
Ângela N MoreiraCentro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil.
Fabricio R ConceiçãoCentro de Desenvolvimento Tecnológico, Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul, Pelotas, Brasil.
Universidade Federal de Pelotas · BRTechnische Universität Braunschweig · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesEarlier studies have demonstrated the use of inactivated recombinant E. coli (bacterins), to protect against Clostridium spp. in vaccinated animals. These bacterins have a simpler, safer, and faster production process. However, these bacterins carry expression plasmids, containing antibiotic resistance gene, which could be assimilate accidentally by environmental microorganisms. Considering this, we aimed to impair this plasmids using formaldehyde at different concentrations.

resultsThis compound inactivated the highest density of cells in 24 h. KanR cassette amplification was found to be impaired with 0.8% for 24 h or 0.4% for 72 h. Upon electroporation, E. coli DH5α ultracompetent cells were unable to acquire the plasmids extracted from the bacterins after inactivation procedure. Formaldehyde-treated bacterins were incubated with other viable strains of E. coli, leading to no detectable gene transfer.

conclusionsWe found that this compound is effective as an inactivation agent. Here we demonstrate the biosafety involving antibiotic resistance gene of recombinant E. coli vaccines allowing to industrial production and animal application.

Indexed as

Escherichia coliEscherichia coli VaccinesFormaldehydeGene Transfer, HorizontalKanamycin ResistancePlasmidsVaccines, InactivatedVaccines, SyntheticEscherichia coli VaccinesFormaldehydeVaccines, InactivatedVaccines, SyntheticAntibiotic resistance geneChemical inactivationRecombinant vaccinesSelection marker

Identifiers

PMID32500473
OpenAlexW3034099935

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.