Structure of Supramers Formed by the Amphiphile Biotin-CMG-DOPE.
Anton Zalygin, Daria Solovyeva, Ivan Vaskan, Stephen Henry, Marcel Schaefer, Pavel Volynsky, Alexander Tuzikov, Elena Korchagina, Ivan Ryzhov, Alexey Nizovtsev and 5 more
Article in ChemistryOpen, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literature
Who cites it
1 citing paper in PubMed, 15 citations in OpenAlex.
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what money
Authors and funding
15 authors at 4 institutions in 2 countries.
Anton ZalyginShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Daria SolovyevaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Ivan VaskanShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Stephen HenrySchool of Engineering Computer & Mathematical Sciences Auckland University of Technology Auckland 1010 New Zealand.
Marcel SchaeferSchool of Engineering Computer & Mathematical Sciences Auckland University of Technology Auckland 1010 New Zealand.
Pavel VolynskyShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Alexander TuzikovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Elena KorchaginaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Ivan RyzhovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Alexey NizovtsevShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Konstantin MochalovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Roman EfremovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Eleonora ShtykovaShubnikov Institute of Crystallography of Federal Scientific Research Centre 'Crystallography and Photonics' of Russian Academy of Sciences Moscow 119333 Russian Federation.
Vladimir OleinikovShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.
Nicolai BovinShemyakin-Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences 16/10 Miklukho-Maklaya str. Moscow 117997 Russia.ORCID 0000-0001-8669-4477
Institute of Bioorganic Chemistry · RUAuckland University of Technology · NZNational Research University Higher School of Economics · RURussian Academy of Sciences · RU
Funding
No grant is acknowledged in the PubMed record.
6 · The paper itself
Abstract
The synthetic function-spacer-lipid (FSL) amphiphile biotin-CMG-DOPE is widely used for delicate ligation of living cells with biotin residues under physiological conditions. Since this molecule has an "apolar-polar-hydrophobic" gemini structure, the supramolecular organization is expected to differ significantly from the classical micelle. Its organization is investigated with experimental methods and molecular dynamics simulations (MDS). Although the linear length of a single biotin-CMG-DOPE molecule is 9.5 nm, the size of the dominant supramer globule is only 14.6 nm. Investigations found that while the DOPE tails form a hydrophobic core, the polar CMG spacer folds back upon itself and predominantly places the biotin reside inside the globule or planar layer. MDS demonstrates that <10 % of biotin residues on the highly water dispersible globules and only 1 % of biotin residues in layer coatings are in an linear conformation and exposing biotin into the aqueous medium. This explains why in biotin-CMG-DOPE apolar biotin residues both in water dispersible globules and coatings on solid surfaces are still capable of interacting with streptavidin.
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
Structure of Supramers Formed by the Amphiphile Biotin-CMG-DOPE. · full record | OpenQuestion