ArticleBiomedical optics express2020
Label-free assessment of hemodynamics in individual cortical brain vessels using third harmonic generation microscopy.
Article in Biomedical optics express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- ECgo: All-optical induction of single endothelial cell injury and capillary occlusion in the brain.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Simultaneous three-photon and optical coherence microscopy deep within an intact mouse brain.Npj imaging · 2026Article
- Live imaging of vascular dynamics in mouse skin.The Journal of investigative dermatology · 2026Article
- Label-free tracking of subcortical white matter degradationBiomedical optics express · 2026Article
- Diurnal differences in cerebral microvascular blood flow and oxygen delivery across brain regions in awake mice.Neurophotonics · 2026Article
- Memory deficits in hypertensive ApoE4 mice reversed by P2Y12 inhibition via different mechanisms in males and perimenopausal females.Research square · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- Label-Free Multiphoton Imaging Reveals Volumetric Shifts Across Development in Sensory-Related Brain Regions of a Miniature Transparent Vertebrate.The Journal of comparative neurology · 2025Article
- Pia-FLOW: Deciphering hemodynamic maps of the pial vascular connectome and its response to arterial occlusion.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cordbioRxiv : the preprint server for biology · 2024Article
- Label-free, whole-brain in vivo mapping in an adult vertebrate with third harmonic generation microscopy.The Journal of comparative neurology · 2024Article
- Single source CARS-based multimodal microscopy system for biological tissue imaging [Invited].Biomedical optics express · 2024Article
- Calcium transients in nNOS neurons underlie distinct phases of the neurovascular response to barrel cortex activation in awake mice.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2023Article
- Differential reductions in the capillary red-blood-cell flux between retina and brain under chronic global hypoperfusion.Neurophotonics · 2023Article
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- Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation.Cell reports methods · 2022Article
- Quantitative Hemodynamic Measurements in Cortical Vessels Using Functional Ultrasound Imaging.Frontiers in neuroscience · 2022Article
- Recent advances in nonlinear microscopy: Deep insights and polarized revelations.The international journal of biochemistry & cell biology · 2021Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
We show that third harmonic generation (THG) microscopy using a 1-MHz train of 1,300-nm femtosecond duration laser pulses enabled visualization of the structure and quantification of flow speed in the cortical microvascular network of mice to a depth of > 1 mm. Simultaneous three-photon imaging of an intravascular fluorescent tracer enabled us to quantify the cell free layer thickness. Using the label-free imaging capability of THG, we measured flow speed in different types of vessels with and without the presence of an intravascular tracer conjugated to a high molecular weight dextran (2 MDa FITC-dextran, 5% w/v in saline, 100 µl). We found a ∼20% decrease in flow speeds in arterioles and venules due to the dextran-conjugated FITC, which we confirmed with Doppler optical coherence tomography. Capillary flow speeds did not change, although we saw a ∼7% decrease in red blood cell flux with dextran-conjugated FITC injection.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.