Evidence map›Paper›PMID 32497392›Full record

ArticleCurrent protocols in microbiology2020

Vibrio fischeri: Laboratory Cultivation, Storage, and Common Phenotypic Assays.

David G Christensen, Karen L Visick

Abstract read
In one paragraph

Article in Current protocols in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Article
  2. Review
  3. A single point mutation is sufficient to driveJournal of bacteriology · 2025
    Article
  4. Article
  5. Microorganisms · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Functional analysis of cyclic diguanylate-modulating proteins inbioRxiv : the preprint server for biology · 2024
    Article
  12. Journal of bacteriology · 2024
    Review
  13. Article
  14. Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Genetic Manipulation of Vibrio fischeri.Current protocols in microbiology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

David G ChristensenDepartment of Microbiology and Immunology, Loyola University Chicago, Maywood, Illinois.
Karen L VisickDepartment of Microbiology and Immunology, Loyola University Chicago, Maywood, Illinois.

Funding

Host-associated biofilm formation and dispersal mechanismsR35GM130355 · NIGMS · LOYOLA UNIVERSITY CHICAGO · PI Karen L Visick · 2019 to 2026
$3.5M
NIGMS NIH HHS R35 GM130355
6 · The paper itself

Abstract

Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of V. fischeri, as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of V. fischeri. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Growth of V. fischeri from frozen stocks Basic Protocol 2: Growth of V. fischeri in rich, undefined liquid medium Alternate Protocol 1: Growth of V. fischeri in minimal medium Basic Protocol 3: Storage of V. fischeri in frozen stocks Basic Protocol 4: Biofilm assay on solid agar Alternate Protocol 2: Biofilm assay in shaking liquid culture Alternate Protocol 3: Biofilm assay in static liquid culture Basic Protocol 5: Motility assay Basic Protocol 6: Luminescence assay.

Indexed as

Aliivibrio fischeriAnti-Bacterial AgentsBacteriological TechniquesBiofilmsCulture MediaLaboratoriesPhenotypePreservation, BiologicalAnti-Bacterial AgentsCulture Mediabacterial cultivationbiofilmluminescencemotilitysymbiosisvibrioVibrio fischeri

Identifiers

PMID32497392
PMCPMC7337994

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.