ArticlePloS one2020
Novel markers for high-throughput protoplast-based analyses of phytohormone signaling.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Symbiont effectors modulate plant signaling to increase host stress resilience.Plant physiology · 2026Article
- A multiplex hextuple luciferase assay for advancing cellular signaling analysis in plant cells.Plant communications · 2026Article
- Non-invasive imaging of defence responses in plants.Nature communications · 2026Article
- Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Frontiers in plant science · 2025Article
- Seeing the invisible: Tools to teach and study plant transcriptional responses.Plant physiology · 2024Article
- Integration of multiple stress signals in plants using synthetic Boolean logic gates.Plant physiology · 2023Article
- Symbiont-host interactome mapping reveals effector-targeted modulation of hormone networks and activation of growth promotion.Nature communications · 2023Article
- NERNST: a genetically-encoded ratiometric non-destructive sensing tool to estimate NADP(H) redox status in bacterial, plant and animal systems.Nature communications · 2023Article
- Hormonal control of promoter activities of Cannabis sativa prenyltransferase 1 and 4 and salicylic acid mediated regulation of cannabinoid biosynthesis.Scientific reports · 2023Article
- Rapid Investigation of Functional Roles of Genes in Regulation of Leaf Senescence Using Arabidopsis Protoplasts.Frontiers in plant science · 2022Article
- Biosensors: A Sneak Peek into Plant Cell's Immunity.Life (Basel, Switzerland) · 2021Review
- Hormones as go-betweens in plant microbiome assembly.The Plant journal : for cell and molecular biology · 2021Review
- Mediator Subunits MED16, MED14, and MED2 Are Required for Activation of ABRE-Dependent Transcription in Arabidopsis.Frontiers in plant science · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Phytohormones mediate most diverse processes in plants, ranging from organ development to immune responses. Receptor protein complexes perceive changes in intracellular phytohormone levels and trigger a signaling cascade to effectuate downstream responses. The in planta analysis of elements involved in phytohormone signaling can be achieved through transient expression in mesophyll protoplasts, which are a fast and versatile alternative to generating plant lines that stably express a transgene. While promoter-reporter constructs have been used successfully to identify internal or external factors that change phytohormone signaling, the range of available marker constructs does not meet the potential of the protoplast technique for large scale approaches. The aim of our study was to provide novel markers for phytohormone signaling in the Arabidopsis mesophyll protoplast system. We validated 18 promoter::luciferase constructs towards their phytohormone responsiveness and specificity and suggest an experimental setup for high-throughput analyses. We recommend novel markers for the analysis of auxin, abscisic acid, cytokinin, salicylic acid and jasmonic acid responses that will facilitate future screens for biological elements and environmental stimuli affecting phytohormone signaling.
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