ArticlePloS one2020
Overexpression of Medicago sativa LEA4-4 can improve the salt, drought, and oxidation resistance of transgenic Arabidopsis.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 28 citations in OpenAlex.
- Genome-Wide Identification, Expression and Tissue-Specific Epigenetic Modification Analysis of theBiology · 2026Article
- Overexpression of the tomato SlLEA_2-26 gene enhances the tolerance to drought and salt stresses in Arabidopsis thaliana.Journal of plant research · 2026Article
- Identification and characterization of LEA gene family in physic nut and functional analysis ofFrontiers in plant science · 2026Article
- Stress Regulatory capabilities discovered in late embryogenesis abundant (LEA) proteins expressed during pecan (Carya illinoinensis) kernel development.BMC genomics · 2025Article
- A Cold-Induced LEA3 Protein, DohD, Confers Cryoprotective Protection Against Low-Temperature Stress inInternational journal of molecular sciences · 2025Article
- ThePlants (Basel, Switzerland) · 2025Article
- Overexpression ofPlants (Basel, Switzerland) · 2025Article
- Identification of potential molecular markers for detection of lengthy chilled storage ofHeliyon · 2024Article
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- Article
- The Late Embryogenesis Abundant Proteins in Soybean: Identification, Expression Analysis, and the Roles of GmLEA4_19 in Drought Stress.International journal of molecular sciences · 2023Article
- An intrinsically disordered region-containing protein mitigates the drought-growth trade-off to boost yields.Plant physiology · 2023Article
- CPR5-mediated nucleo-cytoplasmic localization of IAA12 and IAA19 controls lateral root development during abiotic stress.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Characterization of a NovelFrontiers in plant science · 2022Article
- Integrated GWAS and transcriptomic analysis reveal the candidate salt-responding genes regulating NaFrontiers in plant science · 2022Article
- Transcriptomic analysis of COFrontiers in plant science · 2022Article
- Article
- Late embryogenesis abundant (LEA) gene family inPlant signaling & behavior · 2021Article
- Genome-Wide Association Analysis Coupled With Transcriptome Analysis Reveals Candidate Genes Related to Salt Stress in Alfalfa (Frontiers in plant science · 2021Article
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9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Late embryogenesis abundant (LEA) proteins are widely involved in many adverse conditions among plants. In this study, we isolated a LEA4 gene from alfalfa (Medicago sativa L.) termed MsLEA4-4 via a homology cloning strategy. MsLEA4-4 encodes 166 amino acids, and the structural analysis showed that the gene contained five repeating TAQAAKEKTQQ amino acid motifs. There were a large number of α-helix in MsLEA4-4, and belongs to hydrophilic amino acid. Subcellular localization analysis showed that MsLEA4-4 was localized in the nucleus. The MsLEA4-4 promoter consisted of G-box and A-box elements, abscisic acid-responsive elements (ABREs), photo regulation and photoperiodic-controlling cis-acting elements, and endosperm expression motifs. The MsLEA4-4 overexpressing in Arabidopsis conferred late-germination phenotypes. Resistance of the overexpressed plants to abiotic stress significantly outperformed the wild-type (WT) plants. Under salt stress and abscisic acid treatment, with more lateral roots and higher chlorophyll content, the overexpressed plants has a higher survival rate measured against WT. Compared to those in the WT plants, the levels of soluble sugar and the activity of various antioxidant enzymes were elevated in the overexpressed plants, whereas the levels of proline and malondialdehyde were significantly reduced. The expression levels of several genes such as ABF3, ABI5, NCED5, and NCED9 increased markedly in the overexpressed plants compared to the WT under osmotic stress.
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