ArticleNucleic acids research2020
A universal method for the rapid isolation of all known classes of functional silencing small RNAs.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 62 citations in OpenAlex.
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- Retrovirus insertions in host transcripts trigger de novo piRNA immunity.The EMBO journal · 2026Article
- A reference genome for cultured Drosophila ovarian somatic cells enables studies of transposon and piRNA biology.Genome biology · 2026Article
- The crosstalk network of non-coding RNAs: Emerging opportunities for the clinical application of osteoporosis.Non-coding RNA research · 2026Review
- Co-evolving infectivity and expression patterns drive the diversification of endogenous retroviruses.The EMBO journal · 2026Article
- Germline fate determination by a single ARGONAUTE protein inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Foliar-applied double-stranded RNA is mobile, transfers to plant pathogens, and triggers RNAi.Nucleic acids research · 2026Article
- Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification.PLoS biology · 2026Article
- The MIR157-SPL15 module regulates flowering and inflorescence development in Arabidopsis thaliana under short days and in Arabis alpina.PLoS genetics · 2025Article
- Binding of heterochromatin protein Rhino to a subset of piRNA clusters depends on a combination of two histone marks.Nature structural & molecular biology · 2025Article
- Hyssopus officinalis: MicroRNA-mediated regulation of terpenoid biosynthesis and defense metabolites.Planta · 2025Article
- Optimizing dsRNA sequences for RNAi in pest control and research with the dsRIP web platform.BMC biology · 2025Article
- Atypical epigenetic and small RNA control of degenerated transposons and their fragments in clonally reproducingGenome research · 2025Article
- Escalation of genome defense capacity enables control of an expanding meiotic driver.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Enhanced RNAi does not provide efficient innate antiviral immunity in mice.Nucleic acids research · 2025Article
- PBOX-sRNA-seq uncovers novel features of miRNA modification and identifies selected 5'-tRNA fragments bearing 2'-O-modification.Nucleic acids research · 2024Article
- Evolutionary adaptation of an HP1-protein chromodomain integrates chromatin and DNA sequence signals.eLife · 2024Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diverse classes of silencing small (s)RNAs operate via ARGONAUTE-family proteins within RNA-induced-silencing-complexes (RISCs). Here, we have streamlined various embodiments of a Q-sepharose-based RISC-purification method that relies on conserved biochemical properties of all ARGONAUTEs. We show, in multiple benchmarking assays, that the resulting 15-min benchtop extraction procedure allows simultaneous purification of all known classes of RISC-associated sRNAs without prior knowledge of the samples-intrinsic ARGONAUTE repertoires. Optimized under a user-friendly format, the method - coined 'TraPR' for Trans-kingdom, rapid, affordable Purification of RISCs - operates irrespectively of the organism, tissue, cell type or bio-fluid of interest, and scales to minute amounts of input material. The method is highly suited for direct profiling of silencing sRNAs, with TraPR-generated sequencing libraries outperforming those obtained via gold-standard procedures that require immunoprecipitations and/or lengthy polyacrylamide gel-selection. TraPR considerably improves the quality and consistency of silencing sRNA sample preparation including from notoriously difficult-to-handle tissues/bio-fluids such as starchy storage roots or mammalian plasma, and regardless of RNA contaminants or RNA degradation status of samples.
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