ArticleActa crystallographica. Section D, Structural biology2020
Current approaches for automated model building into cryo-EM maps using Buccaneer with CCP-EM.
Article in Acta crystallographica. Section D, Structural biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Structural visualization of the molecular evolution of CRISPR-Cas9.Nature structural & molecular biology · 2026Article
- Impurities in amyloid studies: The power of automated model building within a cautionary tale for structural biologists.Protein science : a publication of the Protein Society · 2025Article
- A multichaperone condensate enhances protein folding in the endoplasmic reticulum.Nature cell biology · 2025Article
- Structural insights into RNA-guided RNA editing by the Cas13b-ADAR2 complex.Nature structural & molecular biology · 2025Article
- Deciphering the allosteric regulation of mycobacterial inosine-5'-monophosphate dehydrogenase.Nature communications · 2024Article
- DeepMainmast: integrated protocol of protein structure modeling for cryo-EM with deep learning and structure prediction.Nature methods · 2024Article
- Dual function of OmpM as outer membrane tether and nutrient uptake channel in diderm Firmicutes.Nature communications · 2023Article
- Automated model building and protein identification in cryo-EM maps.bioRxiv : the preprint server for biology · 2023Article
- DoubleHelix: nucleic acid sequence identification, assignment and validation tool for cryo-EM and crystal structure models.Nucleic acids research · 2023Article
- Large language models generate functional protein sequences across diverse families.Nature biotechnology · 2023Article
- Article
- Mechanism of protein-primed template-independent DNA synthesis by Abi polymerases.Nucleic acids research · 2022Article
- Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism.The EMBO journal · 2022Article
- ModelCraft: an advanced automated model-building pipeline using Buccaneer.Acta crystallographica. Section D, Structural biology · 2022Article
- Sequence-assignment validation in cryo-EM models with checkMySequence.Acta crystallographica. Section D, Structural biology · 2022Article
- Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.Annual review of biochemistry · 2022Review
- The structure of EXTL3 helps to explain the different roles of bi-domain exostosins in heparan sulfate synthesis.Nature communications · 2022Article
- Structure of the type V-C CRISPR-Cas effector enzyme.Molecular cell · 2022Article
- The human SKI complex regulates channeling of ribosome-bound RNA to the exosome via an intrinsic gatekeeping mechanism.Molecular cell · 2022Article
- CryoEM analysis of small plant biocatalysts at sub-2 Å resolution.Acta crystallographica. Section D, Structural biology · 2022Article
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3 authors.
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Abstract
This work focuses on the use of the existing protein-model-building software Buccaneer to provide structural interpretation of electron cryo-microscopy (cryo-EM) maps. Originally developed for application to X-ray crystallography, the necessary steps to optimise the usage of Buccaneer with cryo-EM maps are shown. This approach has been applied to the data sets of 208 cryo-EM maps with resolutions of better than 4 Å. The results obtained also show an evident improvement in the sequencing step when the initial reference map and model used for crystallographic cases are replaced by a cryo-EM reference. All other necessary changes to settings in Buccaneer are implemented in the model-building pipeline from within the CCP-EM interface (as of version 1.4.0).
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