Evidence map›Paper›PMID 32496044›Full record

ArticleACS synthetic biology2020

Development of Novel Riboswitches for Synthetic Biology in the Green Alga Chlamydomonas.

Payam Mehrshahi, Ginnie Trinh D T Nguyen, Aleix Gorchs Rovira, Andrew Sayer, Marcel Llavero-Pasquina, Michelle Lim Huei Sin, Elliot J Medcalf, Gonzalo I Mendoza-Ochoa, Mark A Scaife, Alison G Smith

Open access · hybridAbstract read
In one paragraph

Article in ACS synthetic biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.0field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 51 citations in OpenAlex.

  1. Review
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  9. EngineeringmLife · 2023
    Article
  10. Review
  11. Article
  12. Article
  13. Recent advances in RNA structurome.Science China. Life sciences · 2022
    Review
  14. Review
  15. Review
  16. Biocircuits in plants and eukaryotic algae.Frontiers in plant science · 2022
    Review
  17. Article
  18. Article
  19. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Payam MehrshahiDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.ORCID 0000-0002-7192-0942
Ginnie Trinh D T NguyenGlanbia Performance Nutrition Canada Inc., 3500 Lacey Road, Suite 1200, Downers Grove, Illinois 60515, United States.
Aleix Gorchs RoviraDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Andrew SayerDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Marcel Llavero-PasquinaDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Michelle Lim Huei SinJohn Swire & Sons (H.K.) Ltd. 33/F One Pacific Place, 88 Queensway, Hong Kong, China.
Elliot J MedcalfDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Gonzalo I Mendoza-OchoaDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.
Mark A ScaifeMara Renewables Corporation, 101A Research Drive, Dartmouth, Nova Scotia B2Y 4T6, Canada.
Alison G SmithDepartment of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, U.K.ORCID 0000-0001-6511-5704
University of Cambridge · GBMara Renewables (Canada) · CA

Funding

Biotechnology and Biological Sciences Research Council BB/D005817/1Biotechnology and Biological Sciences Research Council BB/D006104/1Biotechnology and Biological Sciences Research Council BB/D011043/1Biotechnology and Biological Sciences Research Council BB/I007660/1Biotechnology and Biological Sciences Research Council BB/L014130/1Biotechnology and Biological Sciences Research Council BB/M018210/01
6 · The paper itself

Abstract

Riboswitches are RNA regulatory elements that bind specific ligands to control gene expression. Because of their modular composition, where a ligand-sensing aptamer domain is combined with an expression platform, riboswitches offer unique tools for synthetic biology applications. Here we took a mutational approach to determine functionally important nucleotide residues in the thiamine pyrophosphate (TPP) riboswitch in the THI4 gene of the model alga

Indexed as

5' Untranslated RegionsAlgal ProteinsAptamers, NucleotideChlamydomonasGene ExpressionMetabolic EngineeringMutagenesisRiboswitchThiamine PyrophosphateUltraviolet Rays5' Untranslated RegionsAlgal ProteinsAptamers, NucleotideRiboswitchThiamine PyrophosphateChlamydomonasditerpenoidmetabolic engineeringmicroalgaeriboswitchsynthetic biology

Identifiers

PMID32496044
PMCPMC7309327
OpenAlexW3030823020

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.