ArticleRice (New York, N.Y.)2020
Agrobacterium-Mediated Genetic Transformation of Wild Oryza Species Using Immature Embryos.
Article in Rice (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Overexpression of cryIAb gene for enhancing yellow stem borer (Scirpophaga incertulas) resistance using a marker-free double T-DNA system in Rojolele of Indonesia Rice cultivar.Journal, genetic engineering & biotechnology · 2026Article
- Overexpression ofGenes · 2026Article
- The genetic and developmental enigma of rhizomes: crucial traits with limited understanding.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- Unlocking the potential of wild rice to bring missing nutrition to elite grains.Plant communications · 2025Review
- Usage of wildBreeding science · 2025Article
- Speed breeding: protocols, application and achievements.Frontiers in plant science · 2025Review
- Innovative opportunities for gene editing technology in crop breeding: from the perspective of literature analysis.Frontiers in plant science · 2025Article
- Article
- Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023Review
- Establishment of Agrobacterium-mediated transformation system to Juglans sigillata Dode 'Qianhe-7'.Transgenic research · 2023Article
- Can the Wild Perennial, Rhizomatous Rice Species Oryza longistaminata be a Candidate for De Novo Domestication?Rice (New York, N.Y.) · 2023Review
- Genetic basis controlling rice plant architecture and its modification for breeding.Breeding science · 2023Article
- Overexpression of maize GOLDEN2 in rice and maize calli improves regeneration by activating chloroplast development.Science China. Life sciences · 2023Article
- Challenges and prospects in using biotechnological interventions inGM crops & food · 2022Article
- Protocol for genome editing in wild allotetraploid riceSTAR protocols · 2022Article
- Measurements of Antibacterial Activity of Seed Crude Extracts in Cultivated Rice and Wild Oryza Species.Rice (New York, N.Y.) · 2022Article
- The role of polyplexes in developing a green sustainable approach in agriculture.RSC advances · 2022Review
- Ectopic expression ofBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Establishment of an efficient regeneration andFrontiers in genetics · 2022Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Genetic transformation is one of the most important technologies for revealing or modulating gene function. It is used widely in both functional genomics and molecular breeding of rice. Demands on its use in wild Oryza species is increasing because of their high genetic diversity. Given the difficulties in genetic crosses between distantly related species, genetic transformation offers a way to alter or transfer genetic traits in wild rice accessions. However, transformation of wild Oryza accessions by conventional methods using calli induced from scutellum tissue of embryos in mature seeds often fails. Here, we report methods using immature embryos for the genetic transformation of a broad range of Oryza species. First, we investigated the ability of callus induction and regeneration from immature embryos of 192 accessions in 20 species under several culture conditions. We regenerated plants from immature embryos of 90 accessions in 16 species. Next, we optimized the conditions of Agrobacterium infection using a vector carrying the GFP gene driven by the maize ubiquitin promoter. GFP signals were observed in 51 accessions in 11 species. We analyzed the growth and seed set of transgenic plants of O. barthii, O. glumaepatula, O. rufipogon, and O. brachyantha. The plants grew to maturity and set seeds normally. Southern blot analyses using DNA from T
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.