Evidence map›Paper›PMID 32494996›Full record

ArticleBiotechnology letters2020

Efficient CRISPR/Cas9-mediated gene editing in Guangdong small-ear spotted pig cells using an optimized electrotransfection method.

Yan-Yan Wei, Qun-Mei Zhan, Xiang-Xing Zhu, Ai-Fen Yan, Juan Feng, Lian Liu, Jian-Hao Li, Dong-Sheng Tang

Abstract read
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In one paragraph

Article in Biotechnology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 10 citations in OpenAlex.

  1. SCNT and genome editing in Buffalo, the black gold of the tropics.The Journal of reproduction and development · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yan-Yan Wei *Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, 528225, China.
Qun-Mei Zhan *Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, 528225, China.
Xiang-Xing Zhu *Guangdong Provincial Engineering and Technology Research Center for Gene Editing, School of Medical Engineering, Foshan University, Foshan, 528225, China. zhu_xiangxing@126.com.ORCID http://orcid.org/0000-0002-5265-2620
Ai-Fen YanGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, 528225, China.
Juan FengGuangdong Provincial Engineering and Technology Research Center for Gene Editing, School of Medical Engineering, Foshan University, Foshan, 528225, China.
Lian LiuGuangdong Provincial Engineering and Technology Research Center for Gene Editing, School of Medical Engineering, Foshan University, Foshan, 528225, China.
Jian-Hao LiState Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China. jianhao63@sina.com.
Dong-Sheng TangGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, 528225, China. tangdsh@163.com.
Foshan University · CNGuangdong Academy of Agricultural Sciences · CN

Funding

Foshan Science and Technology Innovation Project 2017AG100111Guangdong Foundation and Applied Basic Research Fund 2019A1515110280Guangdong Provincial R&D Project in Key Areas 2018B020203003Guangzhou Science and Technology Planning Project 201707020007National Science and Technology Major Project 2009ZX08010-023B, 2018ZX08010-08BOpen Project of Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding 2018A05
6 · The paper itself

Abstract

objectivesGuangdong Small-ear Spotted (GDSS) pigs are a pig breed native to China that possesses unfortunate disadvantages, such as slow growth rate, low lean-meat percentage, and reduced feed utilization. In contrast to traditional genetic breeding methods with long cycle time and high cost, CRISPR/Cas9-mediated gene editing for the modification of the pig genome can quickly improve production traits, and therefore this technique exhibits important potential in the genetic improvement and resource development of GDSS pigs. In the present study, we aimed to establish an efficient CRISPR/Cas9-mediated gene-editing system for GDSS pig cells by optimizing the electrotransfection parameters, and to realize efficient CRISPR/Cas9-mediated gene editing of GDSS pig cells.

resultsAfter optimization of electrotransfection parameters for the transfection of GDSS pig cells, we demonstrated that a voltage of 150 V and a single pulse with a pulse duration of 20 ms were the optimal electrotransfection parameters for gene editing in these cells. In addition, our study generated GDSS pig single-cell colonies with biallelic mutations in the myostatin (MSTN) gene and insulin-like growth factor 2 (IGF2) intron-3 locus, which play an important role in pig muscle growth and muscle development. The single-cell colonies showed no foreign gene integration or off-target effects, and maintained normal cell morphology and viability. These gene-edited, single-cell colonies can in the future be used as donor cells to generate MSTN- and IGF2-edited GDSS pigs using somatic cell nuclear transfer (SCNT).

conclusionsThis study establishes the foundation for genetic improvement and resource development of GDSS pigs using CRISPR/Cas9-mediated gene editing combined with SCNT.

Indexed as

AnimalsCell LineCRISPR-Cas SystemsElectromagnetic PhenomenaGene EditingInsulin-Like Growth Factor IIMutationMyostatinSelective BreedingSingle-Cell AnalysisSwineTransfectionInsulin-Like Growth Factor IIMyostatinCRISPR/Cas9-mediated gene editingElectrotransfectionGuangdong small-ear spotted (GDSS) pigInsulin-like growth factor 2 (IGF2)Myostatin (MSTN)

Identifiers

PMID32494996
OpenAlexW3031477063

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.