Evidence map›Paper›PMID 32494742›Full record

ArticleScience advances2020

Combinatorial screening of biochemical and physical signals for phenotypic regulation of stem cell-based cartilage tissue engineering.

Junmin Lee, Oju Jeon, Ming Kong, Amr A Abdeen, Jung-Youn Shin, Ha Neul Lee, Yu Bin Lee, Wujin Sun, Praveen Bandaru, Daniel S Alt and 7 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 1 pooled it
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 8 institutions in 3 countries.

Junmin LeeDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0002-4414-7130
Oju JeonDepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.ORCID 0000-0003-3972-9272
Ming KongCollege of Marine Life Science, Ocean University of China, Yushan Road, Qingdao, Shandong Province 266003, China.
Amr A AbdeenWisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI 53715, USA.ORCID 0000-0003-4071-6620
Jung-Youn ShinDepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.
Ha Neul LeeMolecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Yu Bin LeeDepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.ORCID 0000-0002-1470-6499
Wujin SunDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0002-3167-111X
Praveen BandaruDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0002-8522-7778
Daniel S AltDepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.ORCID 0000-0002-8058-0644
KangJu LeeDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Han-Jun KimDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0001-9238-7238
Sang Jin LeeDepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.ORCID 0000-0002-8007-8785
Somali ChaterjiAgricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.ORCID 0000-0002-3651-6362
Su Ryon ShinDepartment of Medicine, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.ORCID 0000-0003-0864-6482
Eben AlsbergDepartment of Biomedical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA.ORCID 0000-0002-3487-4625
Ali KhademhosseiniDepartment of Bioengineering, Henry Samueli School of Engineering and Applied Sciences, University of California, Los Angeles, Los Angeles, CA 90095, USA.ORCID 0000-0001-6322-8852
California NanoSystems Institute · USUniversity of Illinois Chicago · USBrigham and Women's Hospital · USCase Western Reserve University · USKyung Hee University · KRPurdue University West Lafayette · USUniversity of California, Los Angeles · USUniversity of Wisconsin–Madison · US

Funding

High-Throughput Microenvironment Regulation for ChondrogenesisR01AR066193 · NIAMS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI ALSBERG, EBEN, KHADEMHOSSEINI, ALI · 2015 to 2020
$2.1M
NIAMS NIH HHS R01 AR066193
6 · The paper itself

Abstract

Despite great progress in biomaterial design strategies for replacing damaged articular cartilage, prevention of stem cell-derived chondrocyte hypertrophy and resulting inferior tissue formation is still a critical challenge. Here, by using engineered biomaterials and a high-throughput system for screening of combinatorial cues in cartilage microenvironments, we demonstrate that biomaterial cross-linking density that regulates matrix degradation and stiffness-together with defined presentation of growth factors, mechanical stimulation, and arginine-glycine-aspartic acid (RGD) peptides-can guide human mesenchymal stem cell (hMSC) differentiation into articular or hypertrophic cartilage phenotypes. Faster-degrading, soft matrices promoted articular cartilage tissue formation of hMSCs by inducing their proliferation and maturation, while slower-degrading, stiff matrices promoted cells to differentiate into hypertrophic chondrocytes through Yes-associated protein (YAP)-dependent mechanotransduction. in vitro and in vivo chondrogenesis studies also suggest that down-regulation of the Wingless and INT-1 (WNT) signaling pathway is required for better quality articular cartilage-like tissue production.

Indexed as

Cartilage, ArticularMesenchymal Stem CellsBiocompatible MaterialsCell DifferentiationMechanotransduction, CellularPhenotypeStem CellsTissue EngineeringBiocompatible Materials

Identifiers

PMID32494742
PMCPMC7244269
OpenAlexW3027638145

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.