ArticleScience advances2020
Characterization of imine reductases in reductive amination for the exploration of structure-activity relationships.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 70 citations in OpenAlex.
- Data-driven construction of an imine reductase library capable of broad-scope reductive amination at equimolar substrate concentrations.Nature communications · 2026Article
- Spontaneous Non-Catalyzed Molecular Reactions and Interactions in the Human Body: Biomedical Implications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Nitroreductase-triggered indazole formation.Nature communications · 2026Article
- Cupin-Type Dimethylsulfoniopropionate Lyase from Pelagibacter ubique (DddKAngewandte Chemie (International ed. in English) · 2025Article
- Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases.Synthetic and systems biotechnology · 2025Article
- Expanding the repertoire of imine reductases by mining divergent biosynthetic pathways for promiscuous reactivity.Chem catalysis · 2024Article
- Computational design of an imine reductase: mechanism-guided stereoselectivity reversion and interface stabilization.Chemical science · 2024Article
- Biosynthesis of Diverse Ephedra-Type Alkaloids via a Newly Identified Enzymatic Cascade.Biodesign research · 2024Article
- Biocatalysis in Drug Design: Engineered Reductive Aminases (RedAms) Are Used to Access Chiral Building Blocks with Multiple Stereocenters.Journal of the American Chemical Society · 2023Article
- Structure-Based Design of Small Imine Reductase Panels for Target Substrates.ACS catalysis · 2023Article
- Structure-guided semi-rational design of an imine reductase for enantio-complementary synthesis of pyrrolidinamine.Chemical science · 2023Article
- The Discovery of Imine Reductases and their Utilisation for the Synthesis of Tetrahydroisoquinolines.ChemCatChem · 2023Article
- Actinomycetes-derived imine reductases with a preference towards bulky amine substrates.Communications chemistry · 2022Article
- Multifunctional biocatalysis: An unusual imine reductase.Engineering microbiology · 2022Article
- Reductive aminations by imine reductases: from milligrams to tons.Chemical science · 2022Review
- Article
- New Trends and Future Opportunities in the Enzymatic Formation of C-C, C-N, and C-O bonds.Chembiochem : a European journal of chemical biology · 2022Review
- Article
- Development of Continuous Flow Systems to Access Secondary Amines Through Previously Incompatible Biocatalytic Cascades.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2021Article
- Development of Continuous Flow Systems to Access Secondary Amines Through Previously Incompatible Biocatalytic Cascades*.Angewandte Chemie (International ed. in English) · 2021Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Imine reductases (IREDs) have shown great potential as catalysts for the asymmetric synthesis of industrially relevant chiral amines, but a limited understanding of sequence activity relationships makes rational engineering challenging. Here, we describe the characterization of 80 putative and 15 previously described IREDs across 10 different transformations and confirm that reductive amination catalysis is not limited to any particular subgroup or sequence motif. Furthermore, we have identified another dehydrogenase subgroup with chemoselectivity for imine reduction. Enantioselectivities were determined for the reduction of the model substrate 2-phenylpiperideine, and the effect of changing the reaction conditions was also studied for the reductive aminations of 1-indanone, acetophenone, and 4-methoxyphenylacetone. We have performed sequence-structure analysis to help explain clusters in activity across a phylogenetic tree and to inform rational engineering, which, in one case, has conferred a change in chemoselectivity that had not been previously observed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.