ArticleScience advances2020
Fast widefield imaging of neuronal structure and function with optical sectioning in vivo.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- Polarization-engineered aberration-resilient light sheet microscopy.bioRxiv : the preprint server for biology · 2026Article
- Adaptive optical correction for in vivo two-photon fluorescence microscopy with neural fields.Nature methods · 2026Article
- Optical Approaches to Dissect the Structure and Dynamics of the Synapse at Nanoscale Resolution.Advances in neurobiology · 2026Review
- Voltage imaging with periodic structured illumination.Biomedical optics express · 2026Article
- Widefield Targeted Illumination Microscopy Enables Optically-Sectioned, Motion-Resilient Imaging of Neuronal Fibers and Their Dynamics.Laser & photonics reviews · 2025Article
- Voltage Imaging with Periodic Structured Illumination.bioRxiv : the preprint server for biology · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Optical tweeze-sectioning microscopy for 3D imaging and manipulation of suspended cells.Science advances · 2025Article
- Physics-driven self-supervised learning for fast high-resolution robust 3D reconstruction of light-field microscopy.Nature methods · 2025Article
- Dark-based optical sectioning assists background removal in fluorescence microscopy.Nature methods · 2025Article
- High-speed in vivo calcium recording using structured illumination with self-supervised denoising.Optics continuum · 2024Article
- Neurophotonics beyond the surface: unmasking the brain's complexity exploiting optical scattering.Neurophotonics · 2024Article
- Neurophotonics beyond the Surface: Unmasking the Brain's Complexity Exploiting Optical Scattering.ArXiv · 2024Article
- Article
- Fast, multicolour optical sectioning over extended fields of view with patterned illumination and machine learning.Biomedical optics express · 2024Article
- Tandem aberration correction optics (TACO) in wide-field structured illumination microscopy.Biomedical optics express · 2023Article
- Improving optical sectioning with spinning disk structured illumination microscopy.Optics express · 2023Article
- Deep learning-driven adaptive optics for single-molecule localization microscopy.Nature methods · 2023Article
- Super-Resolution Imaging of Neuronal Structures with Structured Illumination Microscopy.Bioengineering (Basel, Switzerland) · 2023Article
- Superresolution structured illumination microscopy reconstruction algorithms: a review.Light, science & applications · 2023Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Optical microscopy, owing to its noninvasiveness and subcellular resolution, enables in vivo visualization of neuronal structure and function in the physiological context. Optical-sectioning structured illumination microscopy (OS-SIM) is a widefield fluorescence imaging technique that uses structured illumination patterns to encode in-focus structures and optically sections 3D samples. However, its application to in vivo imaging has been limited. In this study, we optimized OS-SIM for in vivo neural imaging. We modified OS-SIM reconstruction algorithms to improve signal-to-noise ratio and correct motion-induced artifacts in live samples. Incorporating an adaptive optics (AO) module to OS-SIM, we found that correcting sample-induced optical aberrations was essential for achieving accurate structural and functional characterizations in vivo. With AO OS-SIM, we demonstrated fast, high-resolution in vivo imaging with optical sectioning for structural imaging of mouse cortical neurons and zebrafish larval motor neurons, and functional imaging of quantal synaptic transmission at
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.