Evidence map›Paper›PMID 32494703›Full record

ArticleScience advances2020

Programming PAM antennae for efficient CRISPR-Cas9 DNA editing.

Fei Wang, Yaya Hao, Qian Li, Jiang Li, Honglu Zhang, Xueli Zhang, Lihua Wang, Carlos Bustamante, Chunhai Fan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. DNA Origami and Its Applications in Synthetic Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  2. Article
  3. Ultra-sensitive biosensor detection of microRNA based on the CRISPR/Cas12a system and exonuclease-assisted target recycling signal amplification.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025
    Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Fei WangJoint Research Center for Precision Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Southern Medical University Affiliated Fengxian Hospital, Shanghai 201499, China.ORCID 0000-0003-3948-6899
Yaya HaoSchool of Chemistry and Chemical Engineering, Frontiers Science Center of Transformative Molecules, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.ORCID 0000-0003-4206-7089
Qian LiSchool of Chemistry and Chemical Engineering, Frontiers Science Center of Transformative Molecules, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.
Jiang LiCAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.ORCID 0000-0003-2372-6624
Honglu ZhangDepartment of Chemistry, Institute for Quantitative Biosciences (QB3), Department of Physics, Department of Molecular and Cell Biology, Kavli Energy Nanosciences Institute at Berkeley, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.
Xueli ZhangJoint Research Center for Precision Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Southern Medical University Affiliated Fengxian Hospital, Shanghai 201499, China.
Lihua WangCAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Carlos BustamanteDepartment of Chemistry, Institute for Quantitative Biosciences (QB3), Department of Physics, Department of Molecular and Cell Biology, Kavli Energy Nanosciences Institute at Berkeley, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA.ORCID 0000-0002-2970-0073
Chunhai FanSchool of Chemistry and Chemical Engineering, Frontiers Science Center of Transformative Molecules, and Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.ORCID 0000-0002-7171-7338

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial CRISPR-Cas9 nucleases have been repurposed as powerful genome editing tools. Whereas engineering guide RNAs or Cas nucleases have proven to improve the efficiency of CRISPR editing, modulation of protospacer-adjacent motif (PAM), indispensable for CRISPR, has been less explored. Here, we develop a DNA origami-based platform to program a PAM antenna microenvironment and address its performance at the single-molecule level with submolecular resolution. To mimic spatially controlled in vivo PAM distribution as may occur in chromatin, we investigate the effect of PAM antennae surrounding target DNA. We find that PAM antennae effectively sensitize the DNA cleavage by recruiting Cas9 molecules. Super-resolution tracking of single single-guide RNA/Cas9s reveals localized translocation of Cas9 among spatially proximal PAMs. We find that the introduction of the PAM antennae effectively modulates the microenvironment for enhanced target cleavage (up to ~50%). These results provide insight into factors that promote more efficient genome editing.

Indexed as

CRISPR-Associated Protein 9CRISPR-Cas SystemsDNAEndonucleasesGene EditingRNA, Guide, CRISPR-Cas SystemsCRISPR-Associated Protein 9DNAEndonucleasesRNA, Guide, CRISPR-Cas Systems

Identifiers

PMID32494703
PMCPMC7209990

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.