ArticleScience advances2020
Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Quantitative evaluation of LED-based optical autofocus module.Journal of microscopy · 2026Article
- Multivalent adaptor networks generate nanoscale organisation within T cell signalling condensates.bioRxiv : the preprint server for biology · 2026Article
- Reinforced optical cage systems enable drift-free single-molecule localization microscopy.Communications engineering · 2025Article
- Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy.Light, science & applications · 2025Article
- Discriminating single-molecule binding events from diffraction-limited fluorescence.Nature communications · 2025Article
- In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy.Nature communications · 2025Article
- Image-based 3D active sample stabilization on the nanometer scale for optical microscopy.Biophysical reports · 2025Article
- Diverse Fluorescent Probe Concepts for Detection and Monitoring of Reactive Oxygen Species.Chemistry, an Asian journal · 2025Review
- Quality control maps: Real-time quantitative control of single-molecule localization microscopy data.Biophysical journal · 2025Article
- The nanoscale organization of the Nipah virus fusion protein informs new membrane fusion mechanisms.eLife · 2025Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.Nature communications · 2024Article
- Super-resolution acoustic displacement metrology through topological pairs in orbital meta-atoms.Nature communications · 2024Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.bioRxiv : the preprint server for biology · 2024Article
- Build and operation of a custom 3D, multicolor, single-molecule localization microscope.Nature protocols · 2024Review
- Membrane topography and the overestimation of protein clustering in single molecule localisation microscopy - identification and correction.Communications biology · 2024Article
- openFrame: A modular, sustainable, open microscopy platform with single-shot, dual-axis optical autofocus module providing high precision and long range of operation.Journal of microscopy · 2023Article
- Advanced imaging techniques for studying protein phase separation in living cells and at single-molecule level.Current opinion in chemical biology · 2023Review
- Tuning axial and lateral localization precision in 3D super-resolution microscopy with variable astigmatism.Optics letters · 2022Article
- Bidirectional feedback between BCR signaling and actin cytoskeletal dynamics.The FEBS journal · 2022Review
- Bleaching-Resistant Super-Resolution Fluorescence Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-molecule localization microscopy (SMLM) has the potential to quantify the diversity in spatial arrangements of molecules in intact cells. However, this requires that the single-molecule emitters are localized with ultrahigh precision irrespective of the sample format and the length of the data acquisition. We advance SMLM to enable direct distance measurements between molecules in intact cells on the scale between 1 and 20 nm. Our actively stabilized microscope combines three-dimensional real-time drift corrections and achieves a stabilization of <1 nm and localization precision of ~1 nm. To demonstrate the biological applicability of the new microscope, we show a 4- to 7-nm difference in spatial separations between signaling T cell receptors and phosphatases (CD45) in active and resting T cells. In summary, by overcoming the major bottlenecks in SMLM imaging, it is possible to generate molecular images with nanometer accuracy and conduct distance measurements on the biological relevant length scales.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.