ArticleScientific reports2020
Analysis of cell-associated DENV RNA by oligo(dT) primed 5' capture scRNAseq.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Single-cell RNA sequencing unravels mosquito biology and host-pathogen interactions.Parasites & vectors · 2026Review
- Molecular and biophysical requirements for B cell receptor-dependent enhancement of dengue virus infection.Journal of virology · 2026Article
- Computational identification of conserved dengue virus envelope protein epitopes for vaccine design and immunodiagnostic platform.Scientific reports · 2026Article
- B Cell Receptor's function in virus entry: Anti-SARS-CoV-2 B cell receptors can mediate viral entry in an ACE2-independent mechanism.PLoS pathogens · 2026Article
- Molecular and biophysical requirements for B cell receptor dependent enhancement of dengue virus infection.bioRxiv : the preprint server for biology · 2026Article
- Innate immune activation profiles are associated with polarization of the T cell response to recombinant arenaviral vectors.Frontiers in systems biology · 2026Article
- Recapitulating dengue virus infection with human pluripotent stem cell-derived liver organoids for antiviral screening.Nature communications · 2025Article
- Advances in Single-Cell Sequencing for Infectious Diseases: Progress and Perspectives.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Single-cell analysis of mature hepatocytes reveals an IRF1-driven restriction of HDV infection.JHEP reports : innovation in hepatology · 2025Article
- Proceedings of the second annual dengue endgame summit: A call to action.PLoS neglected tropical diseases · 2025Article
- Conventional and antibody-enhanced DENV infection of human macrophages induces differential immunotranscriptomic profiles.Journal of virology · 2025Article
- A single-cell atlas of the Culex tarsalis midgut during West Nile virus infection.PLoS pathogens · 2025Article
- Dengue Virus Structural Proteins Are Expressed on the Surface of DENV-Infected Cells and Are a Target for Antibody-Dependent Cellular Phagocytosis.Open forum infectious diseases · 2025Article
- A single-cell atlas of thebioRxiv : the preprint server for biology · 2024Article
- B cell receptor dependent enhancement of dengue virus infection.PLoS pathogens · 2024Article
- Single-cell analysis reveals an antiviral network that controls Zika virus infection in human dendritic cells.Journal of virology · 2024Article
- Single-Cell RNA-Sequencing of RVFV Infection.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Review
- Single-cell RNA-seq methods to interrogate virus-host interactions.Seminars in immunopathology · 2023Review
- Temporally integrated single cell RNA sequencing analysis of PBMC from experimental and natural primary human DENV-1 infections.PLoS pathogens · 2021Article
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Authors and funding
14 authors at 4 institutions in 2 countries.
Funding
Abstract
Dengue is one of the most widespread vector-borne viral diseases in the world. However, the size, heterogeneity, and temporal dynamics of the cell-associated viral reservoir during acute dengue virus (DENV) infection remains unclear. In this study, we analyzed cells infected in vitro with DENV and PBMC from an individual experiencing a natural DENV infection utilizing 5' capture single cell RNA sequencing (scRNAseq). Both positive- and negative-sense DENV RNA was detected in reactions containing either an oligo(dT) primer alone, or in reactions supplemented with a DENV-specific primer. The addition of a DENV-specific primer did not increase the total amount of DENV RNA captured or the fraction of cells identified as containing DENV RNA. However, inclusion of a DENV-specific cDNA primer did increase the viral genome coverage immediately 5' to the primer binding site. Furthermore, while the majority of intracellular DENV sequence captured in this analysis mapped to the 5' end of the viral genome, distinct patterns of enhanced coverage within the DENV polyprotein coding region were observed. The 5' capture scRNAseq analysis of PBMC not only recapitulated previously published reports by detecting virally infected memory and naïve B cells, but also identified cell-associated genomic variants not observed in contemporaneous serum samples. These results demonstrate that oligo(dT) primed 5' capture scRNAseq can detect DENV RNA and quantify virus-infected cells in physiologically relevant conditions, and provides insight into viral sequence variability within infected cells.
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