ArticleScientific reports2020
Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.).
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 43 citations in OpenAlex.
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- Metabolism dynamics in tropical cockroach during a cold-induced recovery period.Biological research · 2025Article
- Endocrine and enzymatic shifts during insect diapause: a review of regulatory mechanisms.Frontiers in physiology · 2025Review
- Management of inorganic elements by overwintering physiology of cold hardy larvae of European corn borer (Ostrinia nubilalis, Hbn.).Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2024Article
- Prolonged Heat Stress during Winter Diapause Alters the Expression of Stress-Response Genes inInternational journal of molecular sciences · 2024Article
- De novo genome assembly of the invasive mosquito species Aedes japonicus and Aedes koreicus.Parasites & vectors · 2023Article
- European Corn Borer (Insects · 2023Article
- Identification of Intrinsically Disordered Proteins and Regions in a Non-Model Insect SpeciesBiomolecules · 2022Article
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- Acclimations to Cold and Warm Conditions Differently Affect the Energy Metabolism of Diapausing Larvae of the European Corn BorerFrontiers in physiology · 2021Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes - citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, -3 °C and -16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.
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