ArticleMicrobial cell factories2020
Obtaining a series of native gradient promoter-5'-UTR sequences in Corynebacterium glutamicum ATCC 13032.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 31 citations in OpenAlex.
- Untranslated region engineering strategies for gene overexpression, fine-tuning, and dynamic regulation.Journal of microbiology (Seoul, Korea) · 2025Review
- Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid inSynthetic and systems biotechnology · 2024Article
- Enhanced cobalamin biosynthesis inSynthetic and systems biotechnology · 2022Article
- Development of a novel platform for recombinant protein production inSynthetic and systems biotechnology · 2022Article
- O-Acetyl-L-homoserine production enhanced by pathway strengthening and acetate supplementation in Corynebacterium glutamicum.Biotechnology for biofuels and bioproducts · 2022Article
- Identification of key genes through the constructed CRISPR-dcas9 to facilitate the efficient production of O-acetylhomoserine inFrontiers in bioengineering and biotechnology · 2022Article
- Construction of an ultra-strong PtacM promoter via engineering the core-element spacer and 5' untranslated region for versatile applications inBiotechnology notes (Amsterdam, Netherlands) · 2022Article
- Identification of Gradient Promoters ofFrontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundCorynebacterium glutamicum is an important industrial microorganism used for the production of many valuable compounds, especially amino acids and their derivatives. For fine-tuning of metabolic pathways, synthetic biological tools are largely based on the rational application of promoters. However, the limited number of promoters make it difficult.
resultsIn this study, according to the analysis of RNA-Seq data, 90 DNA fragments with lengths of 200-500 bp that may contain promoter-5'-UTR (PUTR) sequences were amplified and linked to a fluorescent protein gene. When compared with the common strong PUTR P
conclusionsThese findings demonstrated that the selected PUTRs can stably express different genes, such as the red fluorescence protein gene, and can be useful for fine-tuning regulation of metabolic networks in C. glutamicum or for establishing high-throughput screening strategies through biosensor for the production of useful compounds.
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