Evidence map›Paper›PMID 32493224›Full record

ArticleBMC genomics2020

Distinctions between sex and time in patterns of DNA methylation across puberty.

Sarah Rose Moore, Kathryn Leigh Humphreys, Natalie Lisanne Colich, Elena Goetz Davis, David Tse Shen Lin, Julia Lynn MacIsaac, Michael Steffen Kobor, Ian Henry Gotlib

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Epigenetic regulation of brain development, plasticity, and response to early-life stress.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sarah Rose MooreDepartment of Medical Genetics, University of British Columbia | BC Children's Hospital Research Institute, 938 W 28th Ave, Vancouver, BC, V5Z 4H4, Canada. Sarah.moore@bcchr.ca.ORCID http://orcid.org/0000-0003-4364-9128
Kathryn Leigh HumphreysDepartment of Psychology and Human Development, Vanderbilt University, 230 Appleton Pl, Nashville, TN, 37203, USA.
Natalie Lisanne ColichDepartment of Psychology, University of Washington Seattle, Guthrie Hall (GTH), 119A 98195-1525, Seattle, WA, 98105, USA.
Elena Goetz DavisDepartment of Psychology, Stanford University, 450 Jane Stanford Way, Stanford, CA, 94305, USA.
David Tse Shen LinDepartment of Medical Genetics, University of British Columbia | BC Children's Hospital Research Institute, 938 W 28th Ave, Vancouver, BC, V5Z 4H4, Canada.
Julia Lynn MacIsaacDepartment of Medical Genetics, University of British Columbia | BC Children's Hospital Research Institute, 938 W 28th Ave, Vancouver, BC, V5Z 4H4, Canada.
Michael Steffen KoborDepartment of Medical Genetics, University of British Columbia | BC Children's Hospital Research Institute, 938 W 28th Ave, Vancouver, BC, V5Z 4H4, Canada.
Ian Henry GotlibDepartment of Psychology, Stanford University, 450 Jane Stanford Way, Stanford, CA, 94305, USA.

Funding

Psychobiological Mechanisms Underlying the Association Between Early Life Stress and Depression Across AdolescenceR37MH101495 · NIMH · STANFORD UNIVERSITY · PI IAN H GOTLIB · 2018 to 2026
$6.6M
Neurodevelopmental Mechanisms linking Childhood Adversity with Adolescent Psychopathology: Pubertal Timing and Cortical-Limbic CircuitryF32MH114317 · NIMH · UNIVERSITY OF WASHINGTON · PI COLICH, NATALIE L · 2017 to 2019
$185k
Jacobs Foundation Early Career Fellowship 2017-1261-05NIMH NIH HHS F32 MH114317NIMH NIH HHS F32-MH114317NIMH NIH HHS R37 MH101495NIMH NIH HHS R37MH101495
6 · The paper itself

Abstract

backgroundThere are significant sex differences in human physiology and disease; the genomic sources of these differences, however, are not well understood. During puberty, a drastic neuroendocrine shift signals physical changes resulting in robust sex differences in human physiology. Here, we explore how shifting patterns of DNA methylation may inform these pathways of biological plasticity during the pubertal transition. In this study we analyzed DNA methylation (DNAm) in saliva at two time points across the pubertal transition within the same individuals. Our purpose was to compare two domains of DNAm patterns that may inform processes of sexual differentiation 1) sex related sites, which demonstrated differences between males from females and 2) time related sites in which DNAm shifted significantly between timepoints. We further explored the correlated network structure sex and time related DNAm networks and linked these patterns to pubertal stage, assays of salivary testosterone, a reliable diagnostic of free, unbound hormone that is available to act on target tissues, and overlap with androgen response elements.

resultsSites that differed by biological sex were largely independent of sites that underwent change across puberty. Time-related DNAm sites, but not sex-related sites, formed correlated networks that were associated with pubertal stage. Both time and sex DNAm networks reflected salivary testosterone levels that were enriched for androgen response elements, with sex-related DNAm networks being informative of testosterone levels above and beyond biological sex later in the pubertal transition.

conclusionsThese results inform our understanding of the distinction between sex- and time-related differences in DNAm during the critical period of puberty and highlight a novel linkage between correlated patterns of sex-related DNAm and levels of salivary testosterone.

Indexed as

DNA MethylationGene Regulatory NetworksAdolescentChildEpigenesis, GeneticFemaleGene OntologyHumansMaleOligonucleotide Array Sequence AnalysisPubertySalivaSex CharacteristicsTestosteroneTime FactorsTestosteroneDNA methylationEpigenetic regulationGonadal hormonesPubertySexual differentiationTestosterone

Identifiers

PMID32493224
PMCPMC7268482

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.