ArticlePLoS pathogens2020
Tn-Seq reveals hidden complexity in the utilization of host-derived glutathione in Francisella tularensis.
Article in PLoS pathogens, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Tn-seq of Thermus thermophilus Genome Reveals Unexpected Tolerance to Insertions in Bacterial Common Essential Genes.MicrobiologyOpen · 2026Article
- Host glutathione is required for Rickettsia parkeri cell division and intracellular survival.Nature communications · 2025Article
- Microbial metabolism of host-derived antioxidants.Current opinion in chemical biology · 2025Review
- Genetic screen identifies cell wall enzyme is key for freshwater survival ofbioRxiv : the preprint server for biology · 2024Article
- Generation of tools for expression and purification of the phage-encoded Type I restriction enzyme inhibitor, Ocr.Microbiology (Reading, England) · 2024Article
- Diverse roles of low-molecular weight thiol GSH inCurrent research in microbial sciences · 2024Article
- Tolfenpyrad displaysMicrobiology spectrum · 2023Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Transposon sequencing identifies genes impactingInfection and immunity · 2023Article
- Ribosome heterogeneity results in leader sequence-mediated regulation of protein synthesis inJournal of bacteriology · 2023Article
- Discovery of a glutathione utilization pathway in Francisella that shows functional divergence between environmental and pathogenic species.Cell host & microbe · 2023Article
- Isotope tracing reveals bacterial catabolism of host-derived glutathione during Helicobacter pylori infection.PLoS pathogens · 2023Article
- The glutathione import system satisfies the Staphylococcus aureus nutrient sulfur requirement and promotes interspecies competition.PLoS genetics · 2023Article
- Enabling low-cost and robust essentiality studies with high-throughput transposon mutagenesis (HTTM).PloS one · 2023Article
- A Ribosomal Protein Homolog Governs Gene Expression and Virulence in a Bacterial Pathogen.Journal of bacteriology · 2022Article
- Functional Genomics Identified Novel Genes Involved in Growth at Low Temperatures in Listeria monocytogenes.Microbiology spectrum · 2022Article
- A Resourceful Race: Bacterial Scavenging of Host Sulfur Metabolism during Colonization.Infection and immunity · 2022Review
- The pentose phosphate pathway constitutes a major metabolic hub in pathogenic Francisella.PLoS pathogens · 2021Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
Host-derived glutathione (GSH) is an essential source of cysteine for the intracellular pathogen Francisella tularensis. In a comprehensive transposon insertion sequencing screen, we identified several F. tularensis genes that play central and previously unappreciated roles in the utilization of GSH during the growth of the bacterium in macrophages. We show that one of these, a gene we named dptA, encodes a proton-dependent oligopeptide transporter that enables growth of the organism on the dipeptide Cys-Gly, a key breakdown product of GSH generated by the enzyme γ-glutamyltranspeptidase (GGT). Although GGT was thought to be the principal enzyme involved in GSH breakdown in F. tularensis, our screen identified a second enzyme, referred to as ChaC, that is also involved in the utilization of exogenous GSH. However, unlike GGT and DptA, we show that the importance of ChaC in supporting intramacrophage growth extends beyond cysteine acquisition. Taken together, our findings provide a compendium of F. tularensis genes required for intracellular growth and identify new players in the metabolism of GSH that could be attractive targets for therapeutic intervention.
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