ArticlePloS one2020
One year cross-sectional study in adult and neonatal intensive care units reveals the bacterial and antimicrobial resistance genes profiles in patients and hospital surfaces.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Difference analysis of intestinal microbiota in patients in the intensive care unit using different sampling methods: a systematic review and meta-analysis.Frontiers in microbiology · 2025Pooled it
- Effects of oropharyngeal administration of own mother's milk on oral microbial colonization in very low birth weight infants fed by gastric tube: A randomized controlled trial.Immunity, inflammation and disease · 2024Trial
- Genomic dynamics of antimicrobial resistance transmission between bacteria from intensive care unit surfaces and from critically ill patients.Critical care (London, England) · 2026Article
- Contact-mediated bacterial transmission and infection risk dynamics in a newly opened hospital ward.BMC microbiology · 2026Article
- Durable antimicrobial coatings for hospital sink surfaces using hydrophobized TEMPO-oxidised cellulose nanofibre Pickering emulsions.Scientific reports · 2026Article
- Early detection of nosocomial pathogens in air and surfaces using an innovative genetic approach for surveillance in healthcare settings.Antimicrobial resistance and infection control · 2026Article
- Spatiotemporal characteristics of environmental microbial communities and resistomes in intensive care units of a tertiary hospital in northwest China.Frontiers in microbiology · 2026Article
- Analysis of ICU resistome dynamics in patients, staff and environment for the identification of predictive biomarkers of sepsis and early mortality.Scientific reports · 2025Article
- Critically ill patients will benefit from single isolated laminar-air-flow wards by improving the environmental microbial composition.BMC infectious diseases · 2024Article
- Bacterial Infections: Surveillance, Prevention and Control.Pathogens (Basel, Switzerland) · 2024Article
- 16S rRNA amplicon sequencing and antimicrobial resistance profile of intensive care units environment in 41 Brazilian hospitals.Frontiers in public health · 2024Observational
- A critical assessment of microbial-based antimicrobial sanitizing of inanimate surfaces in healthcare settings.Frontiers in microbiology · 2024Review
- Oral microbiome test as an alternative diagnostic tool for gastric alterations: A prospective, bicentric cross-sectional study.PloS one · 2024Article
- Microbial Community Characterization and Molecular Resistance Monitoring in Geriatric Intensive Care Units in China Using mNGS.Infection and drug resistance · 2023Article
- Clinically relevant pathogens on surfaces display differences in survival and transcriptomic response in relation to probiotic and traditional cleaning strategies.NPJ biofilms and microbiomes · 2022Article
- Prevalence of carbapenem resistance and its potential association with antimicrobial use in humans and animals in rural communities in Vietnam.JAC-antimicrobial resistance · 2022Article
- Ventilation-Associated Particulate Matter Is a Potential Reservoir of Multidrug-Resistant Organisms in Health Facilities.Life (Basel, Switzerland) · 2021Article
- Healthcare-Associated Infections-Related Bacteriome and Antimicrobial Resistance Profiling: Assessing Contamination Hotspots in a Developing Country Public Hospital.Frontiers in microbiology · 2021Article
- Draft Genome Sequences ofMicrobiology resource announcements · 2020Article
- Draft Genome Sequences ofMicrobiology resource announcements · 2020Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several studies have shown the ubiquitous presence of bacteria in hospital surfaces, staff, and patients. Frequently, these bacteria are related to HAI (healthcare-associated infections) and carry antimicrobial resistance (AMR). These HAI-related bacteria contribute to a major public health issue by increasing patient morbidity and mortality during or after hospital stay. Bacterial high-throughput amplicon gene sequencing along with identification of AMR genes, as well as whole genome sequencing (WGS), are biotechnological tools that allow multiple-sample screening for a diversity of bacteria. In this paper, we used these methods to perform a one-year cross sectional profiling of bacteria and AMR genes in adult and neonatal intensive care units (ICU and NICU) in a Brazilian public, tertiary hospital. Our results showed high abundances of HAI-related bacteria such as S. epidermidis, S. aureus, K. pneumoniae, A. baumannii complex, E. coli, E. faecalis, and P. aeruginosa in patients and hospital surfaces. Most abundant AMR genes detected throughout ICU and NICU were mecA, blaCTX-M-1 group, blaSHV-like, and blaKPC-like. We found that NICU environment and patients were more widely contaminated with pathogenic bacteria than ICU. Patient samples, despite the higher bacterial load, have lower bacterial diversity than environmental samples in both units. Finally, we also identified contamination hotspots in the hospital environment showing constant frequencies of bacterial and AMR contamination throughout the year. Whole genome sequencing (WGS), 16S rRNA oligotypes, and AMR identification allowed a high-resolution characterization of the hospital microbiome profile.
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