ArticleMolecular biology of the cell2020
Novel cytokinetic ring components drive negative feedback in cortical contractility.
Article in Molecular biology of the cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Anillin mediates unilateral furrowing during cytokinesis by limiting RhoA binding to its effectors.The Journal of cell biology · 2025Article
- Mechanical and biochemical feedback combine to generate complex contractile oscillations in cytokinesis.Current biology : CB · 2024Article
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- Contractile ring mechanosensation and its anillin-dependent tuning during early embryogenesis.Nature communications · 2023Article
- Anillin forms linear structures and facilitates furrow ingression after septin and formin depletion.Cell reports · 2023Article
- A versatile cortical pattern-forming circuit based on Rho, F-actin, Ect2, and RGA-3/4.The Journal of cell biology · 2022Article
- Rapid assembly of a polar network architecture drives efficient actomyosin contractility.Cell reports · 2022Article
- Modulating RhoA effectors induces transitions to oscillatory and more wavelike RhoA dynamics inMolecular biology of the cell · 2022Article
- Diversity is the spice of life: An overview of how cytokinesis regulation varies with cell type.Frontiers in cell and developmental biology · 2022Review
- Seeing is believing: tools to study the role of Rho GTPases during cytokinesis.Small GTPases · 2022Review
- Cortical excitability and cell division.Current biology : CB · 2021Article
- CYK-1/Formin activation in cortical RhoA signaling centers promotes organismal left-right symmetry breaking.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division.Journal of cell science · 2020Article
- Caenorhabditis elegans septins contribute to the development and structure of the oogenic germline.Cytoskeleton (Hoboken, N.J.)Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Actomyosin cortical contractility drives many cell shape changes including cytokinetic furrowing. While positive regulation of contractility is well characterized, counterbalancing negative regulation and mechanical brakes are less well understood. The small GTPase RhoA is a central regulator, activating cortical actomyosin contractility during cytokinesis and other events. Here we report how two novel cytokinetic ring components, GCK-1 (germinal center kinase-1) and CCM-3 (cerebral cavernous malformations-3), participate in a negative feedback loop among RhoA and its cytoskeletal effectors to inhibit contractility. GCK-1 and CCM-3 are recruited by active RhoA and anillin to the cytokinetic ring, where they in turn limit RhoA activity and contractility. This is evidenced by increased RhoA activity, anillin and nonmuscle myosin II in the cytokinetic ring, and faster cytokinetic furrowing, following depletion of GCK-1 or CCM-3. GCK-1 or CCM-3 depletion also reduced RGA-3 levels in pulses and increased baseline RhoA activity and pulsed contractility during zygote polarization. Together, our results suggest that GCK-1 and CCM-3 regulate cortical actomyosin contractility via negative feedback. These findings have implications for the molecular and cellular mechanisms of cerebral cavernous malformation pathologies.
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