ArticleNucleic acids research2020
One fly-one genome: chromosome-scale genome assembly of a single outbred Drosophila melanogaster.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- The circadian isoform landscape of mouse livers.NAR genomics and bioinformatics · 2026Article
- Telomere-to-telomere genome assembly of the Dipteran Bactrocera dorsalis from a single individual.Nature communications · 2025Article
- Recombinant inbred line panels inform the genetic architecture and interactions of adaptive traits in Drosophila melanogaster.G3 (Bethesda, Md.) · 2025Article
- Generation and analysis of a mouse multitissue genome annotation atlas.Genome research · 2024Article
- Aligning with the 3Rs: alternative models for research into muscle development and inherited myopathies.BMC veterinary research · 2024Review
- AssessingiScience · 2024Article
- Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes.Nature communications · 2024Article
- Single-fly genome assemblies fill major phylogenomic gaps across the Drosophilidae Tree of Life.PLoS biology · 2024Article
- Generation and analysis of a mouse multi-tissue genome annotation atlas.bioRxiv : the preprint server for biology · 2024Article
- Topological structures and syntenic conservation in sea anemone genomes.Nature communications · 2023Article
- Single-fly assemblies fill major phylogenomic gaps across the Drosophilidae Tree of Life.bioRxiv : the preprint server for biology · 2023Article
- Article
- Highly-multiplexed and efficient long-amplicon PacBio and Nanopore sequencing of hundreds of full mitochondrial genomes.BMC genomics · 2023Article
- Chromosome-level genome of the three-spot damselfish, Dascyllus trimaculatus.G3 (Bethesda, Md.) · 2023Article
- High-resolution single-molecule long-fragment rRNA gene amplicon sequencing of bacterial and eukaryotic microbial communities.Cell reports methods · 2023Article
- De novo sequencing, diploid assembly, and annotation of the black carpenter ant, Camponotus pennsylvanicus, and its symbionts by one person for $1000, using nanopore sequencing.Nucleic acids research · 2023Article
- Assembly of a Hybrid Formica aquilonia × F. polyctena Ant Genome From a Haploid Male.The Journal of heredity · 2022Article
- A high-quality de novo genome assembly based on nanopore sequencing of a wild-caught coconut rhinoceros beetle (Oryctes rhinoceros).BMC genomics · 2022Article
- Dynamics and Impacts of Transposable Element Proliferation in the Drosophila nasuta Species Group Radiation.Molecular biology and evolution · 2022Article
- A chromosome-scale genome assembly and karyotype of the ctenophore Hormiphora californensis.G3 (Bethesda, Md.) · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
A high quality genome assembly is a vital first step for the study of an organism. Recent advances in technology have made the creation of high quality chromosome scale assemblies feasible and low cost. However, the amount of input DNA needed for an assembly project can be a limiting factor for small organisms or precious samples. Here we demonstrate the feasibility of creating a chromosome scale assembly using a hybrid method for a low input sample, a single outbred Drosophila melanogaster. Our approach combines an Illumina shotgun library, Oxford nanopore long reads, and chromosome conformation capture for long range scaffolding. This single fly genome assembly has a N50 of 26 Mb, a length that encompasses entire chromosome arms, contains 95% of expected single copy orthologs, and a nearly complete assembly of this individual's Wolbachia endosymbiont. The methods described here enable the accurate and complete assembly of genomes from small, field collected organisms as well as precious clinical samples.
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