ArticleFEMS yeast research2020
The virulence factor urease and its unexplored role in the metabolism of Cryptococcus neoformans.
Article in FEMS yeast research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Molecular Epidemiology, Mating Types, Clinical, and Physiological traits of Microsporum canis in Humans and Companion Animals (Cats and Dogs) in the Guiyang Region, Southwest China.Mycopathologia · 2026Article
- Repositioning Antimicrobial Peptides Against WHO-Priority Fungi.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Article
- Defence Warriors: Exploring the crosstalk between polyamines and oxidative stress during microbial pathogenesis.Redox biology · 2025Review
- Fur-regulated urease contributes to the environmental adaptation ofMicrobiology spectrum · 2025Article
- Cryptococcal nutrient acquisition and pathogenesis: dining on the host.Microbiology and molecular biology reviews : MMBR · 2025Review
- Phosphite inhibitsFrontiers in microbiology · 2025Article
- Mechanisms and Virulence Factors ofJournal of fungi (Basel, Switzerland) · 2024Review
- Review
- Host and fungal factors both contribute to cryptococcosis-associated hyperammonemia (cryptammonia).Microbiology spectrum · 2024Article
- Preclinical Models for Cryptococcosis of the CNS and Their Characterization Using In Vivo Imaging Techniques.Journal of fungi (Basel, Switzerland) · 2024Review
- Exploring polyamine metabolism of the yeast-like fungus, Emergomyces africanus.FEMS yeast research · 2024Article
- First report of environmental isolation of Cryptococcus and Cryptococcus-like yeasts from Boyacá, Colombia.Scientific reports · 2023Article
- Nanotechnology-Based Approaches for Voriconazole Delivery Applied to Invasive Fungal Infections.Pharmaceutics · 2023Review
- Synergistic antifungal interaction ofFrontiers in microbiology · 2023Article
- Macrophage Mediated Immunomodulation DuringFrontiers in cellular and infection microbiology · 2022Review
- HOG1 has an essential role in the stress response, virulence and pathogenicity ofExperimental and therapeutic medicine · 2021Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 5 countries.
Funding
Abstract
Cryptococcal urease is believed to be important for the degradation of exogenous urea that the yeast encounters both in its natural environment and within the human host. Endogenous urea produced by the yeast's own metabolic reactions, however, may also serve as a substrate for the urease enzyme. Using wild-type, urease-deletion mutant and urease-reconstituted strains of Cryptococcus neoformans H99, we studied reactions located up- and downstream from endogenous urea. We demonstrated that urease is important for cryptococcal growth and that, compared to nutrient-rich conditions at 26°C, urease activity is higher under nutrient-limited conditions at 37°C. Compared to cells with a functional urease enzyme, urease-deficient cells had significantly higher intracellular urea levels and also showed more arginase activity, which may act as a potential source of endogenous urea. Metabolic reactions linked to arginase were also affected, since urease-positive and urease-negative cells differed with respect to agmatinase activity, polyamine synthesis, and intracellular levels of proline and reactive oxygen species. Lastly, urease-deficient cells showed higher melanin levels at 26°C than wild-type cells, while the inverse was observed at 37°C. These results suggest that cryptococcal urease is associated with the functioning of key metabolic pathways within the yeast cell.
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