Evidence map›Paper›PMID 32490216›Full record

ArticleBiochemistry and biophysics reports2020

Cloning and mutagenetic modification of the firefly luciferase gene and its use for bioluminescence microscopy of

Katsunori Ogoh, Ryutaro Akiyoshi, Hirobumi Suzuki

Abstract read
In one paragraph

Article in Biochemistry and biophysics reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Mammalian genome research resources available from the National BioResource Project in Japan.Mammalian genome : official journal of the International Mammalian Genome Society · 2024
    Review
  2. Cloning and characterization of luciferase from an Asian firefly Pygoluciola qingyu and its comparison with other beetle luciferases.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024
    Article
  3. Article
  4. Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Katsunori OgohOlympus Corporation, Kuboyama 2-3, Hachioji, Tokyo, 192-8512, Japan.
Ryutaro AkiyoshiOlympus Corporation, Kuboyama 2-3, Hachioji, Tokyo, 192-8512, Japan.
Hirobumi SuzukiOlympus Corporation, Kuboyama 2-3, Hachioji, Tokyo, 192-8512, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bioluminescence microscopy is an area attracting considerable interest in the field of cell biology because it offers several advantages over fluorescence microscopy, including no requirement for excitation light and being phototoxicity free. This method requires brighter luciferase for imaging; however, suitable genetic resource material for this purpose is not available at present. To achieve brighter bioluminescence microscopy, we developed a new firefly luciferase. Using the brighter luciferase, a reporter strain of

Indexed as

Bioluminescence microscopyDrosophila pupaEngrailedFirefly luciferaseGal4-UAS system

Identifiers

PMID32490216
PMCPMC7262549

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.