Evidence map›Paper›PMID 32490060›Full record

ArticleRegenerative therapy2020

Viability evaluation of layered cell sheets after ultraviolet light irradiation of 222 nm.

Nami Hanamura, Hiroyuki Ohashi, Yukihiro Morimoto, Tatsushi Igarashi, Yasuhiko Tabata

Abstract read
In one paragraph

Article in Regenerative therapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Sensitivity Analysis ofPathogens & immunity · 2024
    Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nami HanamuraUshio Inc., Marunouchi 1-6-5, Chiyoda-ku, Tokyo, Japan.
Hiroyuki OhashiUshio Inc., Marunouchi 1-6-5, Chiyoda-ku, Tokyo, Japan.
Yukihiro MorimotoUshio Inc., Marunouchi 1-6-5, Chiyoda-ku, Tokyo, Japan.
Tatsushi IgarashiUshio Inc., Marunouchi 1-6-5, Chiyoda-ku, Tokyo, Japan.
Yasuhiko TabataLaboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Sciences, Kyoto University, 53 Kawara-cho Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThe objective of this study was to evaluate the cell viability of layered cell sheets, irradiated with 222 nm UV light.

methodsUV transmittance of 222 nm and 254 nm was evaluated when the cell sheets of NCTC Clone 929 cells were irradiated UV light. Cell viability was evaluated after irradiation of 222 nm using 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. Following irradiation of two layered cell sheets at 500 mJ/cm

resultsThe UV transmittance of 222 nm was 10 times less than that of 254 nm. A MTT assay revealed that cells of cell sheets irradiated at 222 nm was less damaged than those at 254 nm, when irradiated at 5 mJ/cm

conclusionsIt is concluded that 222 nm irradiation is biologically safe for cell viability.

Indexed as

3D, 3-dementionalCell sheetCellular viabilityCPDs, cyclobutane-pyrimidine dimersH2O2, hydrogen peroxideKr–Cl, krypton-chlorideMTT, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromidePBS, phosphate-buffered salineSD, standard deviationSterilizationUV

Identifiers

PMID32490060
PMCPMC7260610

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.