Evidence map›Paper›PMID 32488675›Full record

ArticleProbiotics and antimicrobial proteins2021

Antifungal Effects of Fusion Puroindoline B on the Surface and Intracellular Environment of Aspergillus flavus.

Ping-Ping Tian, Yang-Yong Lv, Ang Lv, Wen-Jing Yuan, Shuai-Bing Zhang, Na Li, Yuan-Sen Hu

Abstract read
PubMed Publisher
In one paragraph

Article in Probiotics and antimicrobial proteins, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Inhibitory Effect of Puroindoline Peptides onFrontiers in microbiology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Ping-Ping TianCollege of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Yang-Yong LvCollege of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Ang LvCollege of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Wen-Jing YuanCollege of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Shuai-Bing ZhangCollege of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Na LiCollege of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
Yuan-Sen HuCollege of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China. hys308@126.com.ORCID 0000-0002-5611-8783
Henan University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Aspergillus flavus infection is a major issue for safe food storage. In this study, we constructed an efficient prokaryotic expression system for puroindoline B (PINB) protein to detect its antifungal activity. The Puroindoline b gene was cloned into pET-28a (+) vector and expressed in Escherichia coli. Treatment with fusion PINB revealed that it inhibits mycelial growth of A. flavus, a common grain mold. Moreover, fusion PINB-treated A. flavus mycelium withered and exhibited a sunken spore head. As fusion PINB concentration increased, electrical conductivity in mycelium also increased, indicative of cell membrane damage. Furthermore, intracellular malate dehydrogenase and succinate dehydrogenase activity decreased, revealing a disruption in the tricarboxylic acid cycle. Moreover, the dampened activity of the ion pump Na

Indexed as

Antifungal AgentsAspergillus flavusPlant ProteinsRecombinant ProteinsAntifungal AgentsPlant Proteinspuroindoline protein, Triticum aestivumRecombinant ProteinsAspergillus flavusEnergy metabolismOxidative stressPuroindoline BThe nuclear damage

Identifiers

PMID32488675
OpenAlexW3031261224

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.