Evidence map›Paper›PMID 32488441›Full record

ArticleBiotechnology letters2020

Improving the thermostability of a GH97 dextran glucosidase by rational design.

Xiaomin Zhang, Feiyun Chen, Chao He, Wei Fang, Zemin Fang, Xuecheng Zhang, Xiaotang Wang, Yazhong Xiao

Abstract read
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaomin ZhangSchool of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.
Feiyun ChenSchool of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.
Chao HeSchool of Life Sciences, Anhui University, Hefei, 230601, Anhui, China. chaohe@ahu.edu.cn.
Wei FangSchool of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.
Zemin FangSchool of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.
Xuecheng ZhangSchool of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.
Xiaotang WangDepartment of Chemistry and Biochemistry, Florida International University, Miami, FL, 33199, USA.
Yazhong XiaoSchool of Life Sciences, Anhui University, Hefei, 230601, Anhui, China. yzxiao@ahu.edu.cn.ORCID http://orcid.org/0000-0003-2664-742X

Funding

National Natural Science Foundation of China 31570064
6 · The paper itself

Abstract

This study was aimed at improving the thermostability of dextran glucosidase PspAG97A, a member of the glycoside hydrolase family 97, from Pseudoalteromonas sp. K8. A total of 9 lysine residues were chosen using the TKSA-MC program based on the optimization of surface charge-charge interactions and were mutated to glutamate for shifting the enzyme's isoelectric point off its optimum pH value. Three mutants K75E, K363E and K420E showed enhanced thermostability. The triple mutant, K75E/K363E/K420E, was found to be the best with a 7.3-fold increase in half-life (t

Indexed as

Bacterial ProteinsDextransEnzyme StabilityGlycoside HydrolasesHalf-LifeHydrogen-Ion ConcentrationModels, MolecularMutagenesis, Site-DirectedPseudoalteromonasSubstrate SpecificityThermodynamicsBacterial ProteinsDextransGlycoside HydrolasesDextran glucosidaseGH97Isoelectric pointStructural modellingSurface charge–charge interactionsThermostability improving

Identifiers

PMID32488441

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.