ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2020
Simple, sensitive and specific quantification of diamine oxidase activity in complex matrices using newly discovered fluorophores derived from natural substrates.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- First report on whole saliva profiling of Landlly pigs for stress, inflammation, immunity, and systemic homeostasis appraisal: a pilot study.Veterinary research communications · 2025Article
- COVID-19 mRNA-1273 vaccination induced mast cell activation with strongly elevated ThInflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025Article
- Recombinant human diamine oxidase prevents hemodynamic effects of continuous histamine infusion in guinea pigs.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2023Article
- Simple, fast and inexpensive quantification of glycolate in the urine of patients with primary hyperoxaluria type 1.Urolithiasis · 2023Article
- Diamine oxidase knockout mice are not hypersensitive to orally or subcutaneously administered histamine.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2022Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo measure diamine oxidase (DAO) activity with high sensitivity in complex matrices like plasma or tissue extracts radioactive putrescine or horseradish peroxidase (HRP)/hydrogen peroxide (H METHODS AND
resultsCondensation of ortho-aminobenzaldehyde (oABA) with delta-1-pyrroline and delta-1-piperideine, the autocyclization products of the DAO-oxidized natural substrates putrescine and cadaverine, generates new quinazoline fluorophores with absorption and excitation maxima of 430 and 460 nm, respectively, and peak emission at 620 nm. Fluorescent-based detection limits are 20-40 times lower compared to absorption measurements. This assay can be used to measure DAO activity in human plasma after spiking recombinant human (rh)DAO, in rat plasma after intravenous rhDAO administration, in pregnancy plasma and in tissue extracts of DAO wild-type and knock-out mice. Using rat plasma the correlation between rhDAO activity and ELISA data is 99%. Human and rat plasma without DAO spiking and tissue extracts from DAO knock-out mice showed stable and low fluorescence in the presence of high substrate concentrations.
conclusionsIncubation of DAO with the natural substrates putrescine and cadaverine and oABA generates novel fluorophores increasing the detection limit compared to absorption measurements at least tenfold. This simple, sensitive and specific assay allows the non-radioactive quantification of DAO activity in complex matrices like plasma and tissue extracts without interference by antioxidants.
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