ArticleScientific reports2020
Host genotype and exercise exhibit species-level selection for members of the gut bacterial communities in the mouse digestive system.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Short-term high fat diet-induced metabolic endotoxemia in older individuals with obesity: a randomized crossover study.The American journal of clinical nutrition · 2025Trial
- Species- and strain-level assessment usingGut microbesTrial
- Disruption of the gut microbiota in regulator of G protein signaling 14 knockout (RGS14 KO) mice alters the metabolome and reduces enhanced exercise capacity.European journal of applied physiology · 2026Article
- High-Resolution Community Profiling of Active Bacteria and Eukaryotes in Replant-Diseased Blueberry Farm Soils From New Jersey, USA.Environmental microbiology · 2026Article
- Late-in-life treadmill training mitigates gut microbiome imbalances and cardiovascular disease risk in mice.American journal of physiology. Gastrointestinal and liver physiology · 2026Article
- Acute high-intensity exercise alters gut microbiota composition and energy metabolism in different strains of mice.Frontiers in microbiology · 2026Article
- Long-term endurance exercise influences exercise capacity in mice through modulation of the intestinal microbiota by fecal microbiota transplantation.Frontiers in microbiology · 2026Article
- Review
- PGC-1 alpha overexpression in the skeletal muscle results in a metabolically active microbiome which is independent of redox signaling.Scientific reports · 2025Article
- Evaluating the efficiency of 16S-ITS-23S operon sequencing for species level resolution in microbial communities.Scientific reports · 2025Article
- Dehalogenating Desulfoluna spp. are ubiquitous in host-specific sponge microbiomes of the Great Barrier Reef.The ISME journal · 2025Article
- GROND: a quality-checked and publicly available database of full-length 16S-ITS-23S rRNA operon sequences.Microbial genomics · 2024Article
- Assessment of the Safety and Probiotic Properties ofMicroorganisms · 2024Article
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- Biomimetic superabsorbent hydrogel acts as a gut protective dynamic exoskeleton improving metabolic parameters and expanding A. muciniphila.Cell reports. Medicine · 2023Article
- Article
- Synergistic effects of exercise and catalase overexpression on gut microbiome.Environmental microbiology · 2022Article
- A ribosomal operon database and MegaBLAST settings for strain-level resolution of microbiomes.FEMS microbes · 2022Article
- Is Oxford Nanopore sequencing ready for analyzing complex microbiomes?FEMS microbiology ecology · 2021Article
- Early-life effects of juvenile Western diet and exercise on adult gut microbiome composition in mice.The Journal of experimental biology · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mammalian gut microbiome can potentially impact host health and disease state. It is known that the mouse-genome, eating-behavior, and exercise-status promotes higher taxonomic rank-level alterations (e.g. family to phyla-level) of the gut microbiota. Here, host genotype or activity status was investigated to determine if selection of individual bacterial species or strains could be discerned within the murine digestive system. For this study, the fecal bacterial community of adenylyl cyclase 5 knock-out (AC5KO, n = 7) mice or their wild-type (WT, n = 10) littermates under exercise or sedentary conditions were profiled by sequencing rRNA operons. AC5KO mice were chosen since this genotype displays enhanced longevity/exercise capacity and protects against cardiovascular/metabolic disease. Profiling of rRNA operons using the Oxford MinION yielded 65,706 2-D sequences (after size selection of 3.7-5.7 kb) which were screened against an NCBI 16S rRNA gene database. These sequences were binned into 1,566 different best BLAST hits (BBHs) and counted for each mouse sample. Non-metric multidimensional scaling (NMDS) of the gut microbial community demonstrated clustering by physical activity (p = 0.001) but not by host genotype. Additionally, sequence similarity and phylogenetic analysis demonstrated that different bacterial species (closely related to Muribaculum intestinale and Parasutterella excrementihominis) inhabit AC5KO or WT mice depending on activity status. Other bacterial species of the gut microbiota did not follow such patterning (e.g. Turicibacter sanguinis and Turicimonas muris). Our results support the need of improved taxonomic resolution for better characterization of bacterial communities to deepen our understanding of the role of the gut microbiome on host health.
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