ArticleThe Review of scientific instruments2020
A versatile, multi-laser twin-microscope system for light-sheet imaging.
Article in The Review of scientific instruments, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Polarization-engineered aberration-resilient light sheet microscopy.bioRxiv : the preprint server for biology · 2026Article
- Negative-Valence Neurons in the Larval Zebrafish Pallium.bioRxiv : the preprint server for biology · 2026Article
- Contributions of Zebrafish to Our Understanding of the Function and Regulation of Sleep.Current sleep medicine reports · 2026Review
- Acoustic Tweezers for Microscopy of Living Organisms.IEEE transactions on bio-medical engineering · 2025Article
- Neural Basis of Number Sense in Larval Zebrafish.bioRxiv : the preprint server for biology · 2024Article
- More than double the fun with two-photon excitation microscopy.Communications biology · 2024Review
- A single-shot hyperspectral phasor camera for fast, multi-color fluorescence microscopy.Cell reports methods · 2023Article
- First steps into the cloud: Using Amazon data storage and computing with Python notebooks.PloS one · 2023Article
- Extended depth-of-field light-sheet microscopy improves imaging of large volumes at high numerical aperture.Applied physics letters · 2022Article
- Fast whole-brain imaging of seizures in zebrafish larvae by two-photon light-sheet microscopy.Biomedical optics express · 2022Article
- Quantity as a Fish Views It: Behavior and Neurobiology.Frontiers in neuroanatomy · 2022Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Light-sheet microscopy offers faster imaging and reduced phototoxicity in comparison to conventional point-scanning microscopy, making it a preferred technique for imaging biological dynamics for durations of hours or days. Such extended imaging sessions pose a challenge, as it reduces the number of specimens that can be imaged in a given day. Here, we present a versatile light-sheet imaging instrument that combines two independently controlled microscope-twins, built so that they can share an ultrafast near-infrared laser and a bank of continuous-wave visible lasers, increasing the throughput and decreasing the cost. To permit a wide variety of specimens to be imaged, each microscope-twin provides flexible imaging parameters, including (i) operation in one-photon and/or two-photon excitation modes, (ii) delivery of one to three light-sheets via a trio of orthogonal excitation arms, (iii) sub-micron to micron imaging resolution, (iv) multicolor compatibility, and (v) upright (with provision for inverted) detection geometry. We offer a detailed description of the twin-microscope design to aid instrument builders who wish to construct and use similar systems. We demonstrate the instrument's versatility for biological investigation by performing fast imaging of the beating heart in an intact zebrafish embryo, deep imaging of thick patient-derived tumor organoids, and gentle whole-brain imaging of neural activity in behaving larval zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.