Evidence map›Paper›PMID 32486240›Full record

ArticleInternational journal of molecular sciences2020

Functional Heterologous Expression of Mature Lipase LipA from

Ingrid Yamile Pulido, Erlide Prieto, Gilles Paul Pieffet, Lina Méndez, Carlos A Jiménez-Junca

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 24 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Ethanol as additive enhance the performance of immobilized lipase LipA fromBiotechnology reports (Amsterdam, Netherlands) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ingrid Yamile PulidoBiosciences Doctoral Program, Universidad de La Sabana, km 7 Autopista Norte, Chía 250001, Colombia.
Erlide PrietoAgro-industrial Processes Research Group, Engineering Faculty, Universidad de La Sabana, km 7 Autopista Norte, Chía, Cundinamarca 250001, Colombia.
Gilles Paul PieffetScience Faculty, Universidad Antonio Nariño, Calle 58 A # 37-94 Bogotá D.C.111511, Colombia.
Lina MéndezAgro-industrial Processes Research Group, Engineering Faculty, Universidad de La Sabana, km 7 Autopista Norte, Chía, Cundinamarca 250001, Colombia.
Carlos A Jiménez-JuncaBioprospecting Research Group, Engineering Faculty, Universidad de La Sabana, km 7 Autopista Norte, Chía, Cundinamarca 250001, Colombia.
Universidad de La Sabana · COUniversidad Antonio Nariño · CO

Funding

Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS) 647-2014
6 · The paper itself

Abstract

This study aimed to express heterologously the lipase LipA from Pseudomonas aeruginosa PSA01 obtained from palm fruit residues. In previous approaches, LipA was expressed in Escherichia coli fused with its signal peptide and without its disulfide bond, displaying low activity. We cloned the mature LipA with its truncated chaperone Lif in a dual plasmid and overexpressed the enzyme in two E. coli strains: the traditional BL21 (DE3) and the SHuffle

Indexed as

Bacterial ProteinsComputer SimulationCytoplasmDisulfidesEscherichia coliGene Expression ProfilingIndustrial MicrobiologyLactoseLipaseMolecular ChaperonesMolecular Dynamics SimulationPhoeniceaePlasmidsPromoter Regions, GeneticProtein ConformationProtein DomainsBacterial ProteinsDisulfidesLactoseLipaseMolecular ChaperonesProtein Sorting SignalsSolventsexpression of disulfide bond proteins.lipase and foldase overexpressionlipase LipAoverexpression of E. coli SHufflePseudomonas aeruginosa lipase

Identifiers

PMID32486240
PMCPMC7312249
OpenAlexW3029677551

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.