Evidence map›Paper›PMID 32485953›Full record

ArticleInternational journal of molecular sciences2020

Syndecan-1 Facilitates the Human Mesenchymal Stem Cell Osteo-Adipogenic Balance.

Chieh Yu, Ian W Peall, Son H Pham, Rachel K Okolicsanyi, Lyn R Griffiths, Larisa M Haupt

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Syndecans in hematopoietic cells and their niches.American journal of physiology. Cell physiology · 2024
    Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Review
  16. CD137 Regulates Bone LossFrontiers in endocrinology · 2022
    Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Chieh YuQueensland University of Technology, Genomics Research Centre, School of Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.ORCID 0000-0003-3712-5491
Ian W PeallQueensland University of Technology, Genomics Research Centre, School of Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
Son H PhamQueensland University of Technology, Genomics Research Centre, School of Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
Rachel K OkolicsanyiQueensland University of Technology, Genomics Research Centre, School of Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.
Lyn R GriffithsQueensland University of Technology, Genomics Research Centre, School of Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.ORCID 0000-0002-6774-5475
Larisa M HauptQueensland University of Technology, Genomics Research Centre, School of Biomedical Sciences, IHBI, Brisbane, QLD 4059, Australia.ORCID 0000-0002-7735-8110
Queensland University of Technology · AU

Funding

Australian Postgraduate Award CYSchool of Biomedical Sciences, QUT Ph.D. Enabling Grant, Haupt
6 · The paper itself

Abstract

Bone marrow-derived human mesenchymal stems cells (hMSCs) are precursors to adipocyte and osteoblast lineage cells. Dysregulation of the osteo-adipogenic balance has been implicated in pathological conditions involving bone loss. Heparan sulfate proteoglycans (HSPGs) such as cell membrane-bound syndecans (SDCs) and glypicans (GPCs) mediate hMSC lineage differentiation and with syndecan-1 (SDC-1) reported in both adipogenesis and osteogenesis, these macromolecules are potential regulators of the osteo-adipogenic balance. Here, we disrupted the HSPG profile in primary hMSC cultures via temporal knockdown (KD) of SDC-1 using RNA interference (RNAi) in undifferentiated, osteogenic and adipogenic differentiated hMSCs. SDC-1 KD cultures were examined for osteogenic and adipogenic lineage markers along with changes in HSPG profile and common signalling pathways implicated in hMSC lineage fate. Undifferentiated hMSC SDC-1 KD cultures exhibited a pro-adipogenic phenotype with subsequent osteogenic differentiation demonstrating enhanced maturation of osteoblasts. In cultures where SDC-1 KD was performed following initiation of differentiation, increased adipogenic gene and protein marker expression along with increased Oil Red O staining identified enhanced adipogenesis, with impaired osteogenesis also observed in these cultures. These findings implicate SDC-1 as a facilitator of the hMSC osteo-adipogenic balance during early induction of lineage differentiation.

Indexed as

AdipocytesAdipogenesisAdiposityCell DifferentiationCell LineageCell MembraneCell ProliferationExtracellular Matrix ProteinsHeparan Sulfate ProteoglycansHumansMesenchymal Stem CellsOsteoblastsOsteocytesOsteogenesisRNA InterferenceRNA, Small InterferingExtracellular Matrix ProteinsHeparan Sulfate ProteoglycansRNA, Small InterferingSDC1 protein, humanSyndecan-1adipogenesisheparan sulfate proteoglycanslineage fatemesenchymal stem cellsosteogenesissyndecan-1

Identifiers

PMID32485953
PMCPMC7312587
OpenAlexW3032801458

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.