ReviewInternational journal of molecular sciences2020
The Impact of Single- and Double-Strand DNA Breaks in Human Spermatozoa on Assisted Reproduction.
Review in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07621991 (A Prospective Trial Assessing the Impact of Double-strand Breaks on ART Outcomes and Predicting Its Clinical Thresholds), which is not on this map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Prospective Trial Assessing the Impact of Double-strand Breaks on ART Outcomes and Predicting Its Clinical Thresholds
Who cites it
37 citing papers in PubMed.
- Clustering of sperm by chromatin integrity features reveals breed and monthly effects, as well as a link between double-strand DNA breaks and litter size in pigs.BMC veterinary research · 2026Article
- Double-stranded sperm DNA fragmentation measured with neutral comet assay as a predictor of IVF outcomes: evidence from three European clinics in a multi-centred prospective study.Human reproduction (Oxford, England) · 2026Article
- Complex in vitro fertilization laboratory factors affecting sperm DNA integrity: A narrative review.International journal of reproductive biomedicine · 2025Review
- Perspective in the Mechanisms for Repairing Sperm DNA Damage.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Perspectives on biomarkers of reproductive aging for fertility and beyond.Nature aging · 2024Review
- Impact of Microfluidic Sperm Sorting on Embryonic Euploidy in Infertile Patients with Sperm DNA Damage: A Retrospective Study.International journal of fertility & sterility · 2024Article
- Cryopreservation of bovine sperm causes single-strand DNA breaks that are localized in the toroidal regions of chromatin.Journal of animal science and biotechnology · 2024Article
- Oxidative Stress Biomarkers in Male Infertility: Established Methodologies and Future Perspectives.Genes · 2024Review
- Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen-Thawed Horse Sperm.Antioxidants (Basel, Switzerland) · 2024Article
- Role of DNase Activity in Human Sperm DNA Fragmentation.Biomolecules · 2024Review
- Outcomes comparison of testicular versus ejaculated sperm for intracytoplasmic sperm injection in infertile men with high DNA fragmentation: updated systematic review and meta-analysis.Translational andrology and urology · 2023Article
- A Side-by-Side Comparison of Wildtype and Variant Melanocortin 1 Receptor Signaling with Emphasis on Protection against Oxidative Damage to DNA.International journal of molecular sciences · 2023Article
- Biomarkers of aging.Science China. Life sciences · 2023Review
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- A comprehensive investigation of human endogenous retroviral syncytin proteins and their receptors in men with normozoospermia and impaired semen quality.Journal of assisted reproduction and genetics · 2023Article
- Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used.Biological research · 2022Article
- Article
- Determination of double- and single-stranded DNA breaks in bovine sperm is predictive of their fertilizing capacity.Journal of animal science and biotechnology · 2022Article
- Testicular Immunity and Its Connection with the Microbiota. Physiological and Clinical Implications in the Light of Personalized Medicine.Journal of personalized medicine · 2022Review
- TMT-Based Proteomic Analysis of Human Spermatozoa from Unexplained Recurrent Miscarriage Patients before and after Oral Antioxidant Treatment.Biomedicines · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several cellular insults can result in sperm DNA fragmentation either on one or both DNA strands. Oxidative damage, premature interruption of the apoptotic process and defects in DNA compaction during spermatogenesis are the main mechanisms that cause DNA breaks in sperm. The two-tailed Comet assay is the only technique that can differentiate single- (SSBs) from double- (DSBs) strand DNA breaks. Increased levels of the phosphorylated isoform of the H2AX histone are directly correlated with DSBs and proposed as a molecular biomarker of DSBs. We have carried out a narrative review on the etiologies associated with SSBs and DSBs in sperm DNA, their association with reproductive outcomes and the mechanisms involved in their repair. Evidence suggests a stronger negative impact of DSBs on reproductive outcomes (fertilization, implantation, miscarriage, pregnancy, and live birth rates) than SSBs, which can be partially overcome by using intracytoplasmic sperm injection (ICSI). In sperm, SSBs are irreversible, whereas DSBs can be repaired by homologous recombination, non-homologous end joining (NHEJ) and alternative NHEJ pathways. Although few studies have been published, further research is warranted to provide a better understanding of the differential effects of sperm SSBs and DSBs on reproductive outcomes as well as the prognostic relevance of DNA breaks discrimination in clinical practice.
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