ArticlePathogens (Basel, Switzerland)2020
Detection of Foot-and-Mouth Disease Virus in Swine Meat Juice.
Article in Pathogens (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 9 citations in OpenAlex.
- Environmental Sampling Using Shoe Booties for the Detection of Foot and Mouth Disease Virus in Animal Pens.Viruses · 2026Article
- Article
- Article
- Comparison of diaphragm meat juice and muscle swab samples to spleen and spleen swab samples for the detection of African swine fever viral nucleic acid.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Detection of antibodies to structural proteins of foot-and-mouth disease virus in swine meat juice.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2022Article
- Development of reverse-transcriptase, real-time PCR assays to distinguish the Southern African Territories (SAT) serotypes 1 and 3 and topotype VII of SAT2 of Foot-and-Mouth Disease Virus.Frontiers in veterinary science · 2022Article
- Advances in the differential molecular diagnosis of vesicular disease pathogens in swine.Frontiers in microbiology · 2022Review
- Meat Exudate for Detection of African Swine Fever Virus Genomic Material and Anti-ASFV Antibodies.Viruses · 2021Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Foot-and-mouth disease virus (FMDV) is a highly contagious agent that impacts livestock industries worldwide, leading to significant financial loss. Its impact can be avoided or minimized if the virus is detected early. FMDV detection relies on vesicular fluid, epithelial tags, swabs, serum, and other sample types from live animals. These samples might not always be available, necessitating the use of alternative sample types. Meat juice (MJ), collected after freeze-thaw cycles of skeletal muscle, is a potential sample type for FMDV detection, especially when meat is illegally imported. We have performed experiments to evaluate the suitability of MJ for FMDV detection. MJ was collected from pigs that were experimentally infected with FMDV. Ribonucleic acid (RNA) was extracted from MJ, sera, oral swabs, and lymph nodes from the same animals and tested for FMDV by real-time reverse transcription polymerase chain reaction (rRT-PCR). MJ was also tested for FMDV antigen by Lateral Flow Immunoassay (LFI). FMDV RNA was detected in MJ by rRT-PCR starting at one day post infection (DPI) and as late as 21 DPI. In contrast, FMDV RNA was detected in sera at 1-7 DPI. Antigen was also detected in MJ at 1-9 DPI by LFI. Live virus was not isolated directly from MJ, but was recovered from the viral genome by transfection into susceptible cells. The data show that MJ is a good sample type for FMDV detection.
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