ArticleACS chemical biology2020
Multiplexed Photoactivation of mRNA with Single-Cell Resolution.
Article in ACS chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Emerging strategies and innovations in circular RNA synthesis.Molecular therapy. Nucleic acids · 2026Review
- Article
- Optogenetic Tools for Regulating RNA Metabolism and Functions.Chembiochem : a European journal of chemical biology · 2024Review
- RNA-TAG Mediated Protein-RNA Conjugation.Chembiochem : a European journal of chemical biology · 2023Article
- Genetic Encoding of Arylazopyrazole Phenylalanine for Optical Control of Translation.ACS omega · 2023Article
- Orthogonal Light-Activated DNA for Patterned Biocomputing within Synthetic Cells.Journal of the American Chemical Society · 2023Article
- Site-Specific Covalent Labeling of DNA Substrates by an RNA Transglycosylase.Journal of the American Chemical Society · 2023Article
- Light-Activated Translation of Different mRNAs in Cells via Wavelength-Dependent Photouncaging.Angewandte Chemie (International ed. in English) · 2023Article
- Enzymatic Modification of the 5' Cap with Photocleavable ONB-Derivatives Using GlaTgs V34A.Chembiochem : a European journal of chemical biology · 2023Article
- Photocaged 5' cap analogues for optical control of mRNA translation in cells.Nature chemistry · 2022Article
- Site-Specific and Enzymatic Cross-Linking of sgRNA Enables Wavelength-Selectable Photoactivated Control of CRISPR Gene Editing.Journal of the American Chemical Society · 2022Article
- Translational control of gene function through optically regulated nucleic acids.Chemical Society reviews · 2021Review
- Control of RNA with quinone methide reversible acylating reagents.Organic & biomolecular chemistry · 2021Article
- Chemical synthesis of stimuli-responsive guide RNA for conditional control of CRISPR-Cas9 gene editing.Chemical science · 2021Article
- Visible-to-NIR-Light Activated Release: From Small Molecules to Nanomaterials.Chemical reviews · 2020Article
- Controlling gene expression with light: a multidisciplinary endeavour.Biochemical Society transactions · 2020Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We demonstrate sequential optical activation of two types of mRNAs in the same mammalian cell through the sequential photocleavage of small molecule caging groups ("photocages") tethered to the 5'-untranslated region (5'-UTR) of mRNAs. Synthetic photocages were conjugated onto target mRNA using RNA-TAG, an enzymatic site-specific RNA modification technique. Translation of mRNA was severely reduced upon conjugation of the photocages onto the 5'-UTR. However, subsequent photorelease of the cages from the mRNA transcript triggered activation of translation with single-cell spatiotemporal resolution. To achieve sequential photoactivation of two mRNAs in the same cell, we synthesized a pair of photocages that can be selectively cleaved from mRNA upon photoirradiation with different wavelengths of light. Sequential photoactivation of two mRNAs enabled precise optical control of translation of two unique transcripts. We believe that this modular approach to precisely and rapidly control gene expression will serve as a powerful tool in future biological studies that require controlling translation of multiple transcripts with high spatiotemporal resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.