ArticleParasitology2020
Accuracy of real-time polymerase chain reaction to detect
Article in Parasitology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 2 of them syntheses that pooled it.
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Who cites it
18 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Accuracy of diagnostic strategies for detecting Schistosoma mansoni infection in Brazil: A systematic review and meta-analysis.Revista da Sociedade Brasileira de Medicina Tropical · 2026Pooled it
- Changing seroprevalence of schistosomiasis japonica in China from 1982 to 2020: A systematic review and spatial analysis.PLoS neglected tropical diseases · 2024Pooled it
- Comparative evaluation of Sm1-7-qPCR systems incorporated with Maxwell kit-based and NaOH-based DNA extraction methods for the detection of Schistosoma mansoni infection.Infectious diseases of poverty · 2026Article
- Programmable PNA-nanoparticle hybrids as nanoscale recognition architectures for amplification-free nucleic acid recognition.Biosensors & bioelectronics · 2026Article
- Development of an Indirect ELISA and a Microsphere Detection Test Strip for the Detection of Spirometra mansoni.Transboundary and emerging diseases · 2026Article
- Comparative performance of Kato-Katz, POC-CCA and real-time PCR in detecting Schistosoma mansoni infection at different endemicity settings in northwest Ethiopia: a cross-sectional study.Tropical medicine and health · 2025Article
- Seropositive Rate and Associated Factors of Schistosomiasis in Hunan Province, China: A Three-Year Cross-Sectional Survey.Acta parasitologica · 2025Article
- Descriptive study on risk of increased morbidity of schistosomiasis and graft loss after liver transplantation.Revista da Sociedade Brasileira de Medicina Tropical · 2024Article
- Development and evaluation of an indirect ELISA using a multiepitope antigen for the diagnosis of intestinal schistosomiasis.Parasitology · 2023Article
- Two Molecular Plasma-Based Diagnostic Methods to Evaluate Early Infection ofMicroorganisms · 2023Article
- Accuracy Study of Kato-Katz and Helmintex Methods for Diagnosis of Schistosomiasis Mansoni in a Moderate Endemicity Area in Sergipe, Northeastern Brazil.Diagnostics (Basel, Switzerland) · 2023Article
- The Road to Elimination: Current State of Schistosomiasis Research and Progress Towards the End Game.Frontiers in immunology · 2022Review
- Assessment of the accuracy of 11 different diagnostic tests for the detection ofFrontiers in microbiology · 2022Article
- Reliability of point-of-care circulating cathodic antigen assay for diagnosing schistosomiasis mansoni in urine samples from an endemic area of Brazil after one year of storage at -20 degrees Celsius.Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Diagnostic performance of a urine-based ELISA assay for the screening of human schistosomiasis japonica: A comparative study.Frontiers in microbiology · 2022Article
- Previous History of American Tegumentary Leishmaniasis Alters Susceptibility and Immune Response AgainstFrontiers in immunology · 2021Article
- Development of a Recombinase Polymerase Amplification Assay for Schistosomiasis Japonica Diagnosis in the Experimental Mice and Domestic Goats.Frontiers in cellular and infection microbiology · 2021Article
- A Real-Time PCR Assay for the Diagnosis of Intestinal Schistosomiasis and Cure Assessment After the Treatment of Individuals With Low Parasite Burden.Frontiers in immunology · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to the efforts to control schistosomiasis transmission in tropical countries, a large proportion of individuals from endemic areas present low parasite loads, which hinders diagnosis of intestinal schistosomiasis by the Kato-Katz (KK) method. Therefore, the development of more sensitive diagnostic methods is essential for efficient control measures. The aim was to evaluate the accuracy of a real-time polymerase chain reaction (RT-PCR) to detect Schistosoma mansoni DNA in fecal samples of individuals with low parasite loads. A cross-sectional population-based study was conducted in a rural community (n = 257) in Brazil. POC-CCA® was performed in urine and feces were used for RT-PCR. In addition, fecal exams were completed by 18 KK slides, saline gradient and Helmintex techniques. The combined results of the three parasitological tests detected schistosome eggs in 118 participants (45.9%) and composed the consolidated reference standard (CRS). By RT-PCR, 117 out of 215 tested samples were positive, showing 91.4% sensitivity, 80.2% specificity and good concordance with the CRS (kappa = 0.71). RT-PCR identified 86.9% of the individuals eliminating less than 12 eggs/g of feces, demonstrating much better performance than POC-CCA® (50.8%). Our results showed that RT-PCR is a valuable alternative for the diagnosis of intestinal schistosomiasis in individuals with very low parasite loads.
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