ArticleTheranostics2020
Faster, sharper, more precise: Automated Cluster-FLIM in preclinical testing directly identifies the intracellular fate of theranostics in live cells and tissue.
Article in Theranostics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- A Nomogram Based on a Collagen Feature Support Vector Machine for Predicting the Treatment Response to Neoadjuvant Chemoradiotherapy in Rectal Cancer Patients.Annals of surgical oncology · 2021Trial
- Phasor-Based Spatio-Spectral Segmentation for Hyperspectral Fluorescence Microscopy.Journal of biophotonics · 2026Article
- Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.Journal of biomedical optics · 2026Article
- Development and validation of a collagen score-based prediction model for neoadjuvant chemoimmunotherapy efficacy in locally advanced esophageal squamous cell carcinoma.Journal of thoracic disease · 2026Article
- Segmentation-guided photon pooling enables robust single cell analysis and fast fluorescence lifetime imaging microscopy.bioRxiv : the preprint server for biology · 2025Article
- Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Visualization of Nanocarriers and Drugs in Cells and Tissue.Handbook of experimental pharmacology · 2024Article
- QuasAr Odyssey: the origin of fluorescence and its voltage sensitivity in microbial rhodopsins.Nature communications · 2022Article
- In Vitro Models of Biological Barriers for Nanomedical Research.International journal of molecular sciences · 2022Review
- Comparison of fluorescence lifetime and multispectral imaging for quantitative multiplexing in biological tissue.Biomedical optics express · 2022Article
- Improved fluorescent phytochromes for in situ imaging.Scientific reports · 2022Article
- Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Deep learning in macroscopic diffuse optical imaging.Journal of biomedical optics · 2022Review
- Development of a rapid and sensitive quantum dot nanobead-based double-antigen sandwich lateral flow immunoassay and its clinical performance for the detection of SARS-CoV-2 total antibodies.Sensors and actuators. B, Chemical · 2021Article
- A Dual Fluorescence-Spin Label Probe for Visualization and Quantification of Target Molecules in Tissue by Multiplexed FLIM-EPR Spectroscopy.Angewandte Chemie (International ed. in English) · 2021Article
- Phasor-based image segmentation: machine learning clustering techniques.Biomedical optics express · 2021Article
- Luminescence lifetime imaging of three-dimensional biological objects.Journal of cell science · 2021Article
- A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues.Frontiers in physics · 2021Article
- Skin Barriers in Dermal Drug Delivery: Which Barriers Have to Be Overcome and How Can We Measure Them?Pharmaceutics · 2020Review
- Multiplexed non-invasive tumor imaging of glucose metabolism and receptor-ligand engagement using dark quencher FRET acceptor.Theranostics · 2020Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence microscopy is widely used for high content screening in 2D cell cultures and 3D models. In particular, 3D tissue models are gaining major relevance in modern drug development. Enabling direct multiparametric evaluation of complex samples, fluorescence lifetime imaging (FLIM) adds a further level to intensity imaging by the sensitivity of the fluorescence lifetime to the microenvironment. However, the use of FLIM is limited amongst others by the acquisition of sufficient photon numbers without phototoxic effects in live cells. Herein, we developed a new cluster-based analysis method to enhance insight, and significantly speed up analysis and measurement time for the accurate translation of fluorescence lifetime information into pharmacological pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.