ArticleNature chemical biology2020
Selective N-glycan editing on living cell surfaces to probe glycoconjugate function.
Article in Nature chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 63 citations in OpenAlex.
- N-linked glycosylation of GETV envelope proteins affect viral infection and pathogenicity.Virulence · 2026Article
- Allosteric activation of RNF20/RNF40-RAD6A-mediated H2BK120 monoubiquitylation by H2BS112 GlcNAcylation.Nature chemical biology · 2026Article
- Nanopore profiling and structure determination of oligosaccharides by fragmentation.Science advances · 2026Article
- Altered Cell SurfaceJournal of proteome research · 2025Article
- Facile expression of proteins with desired N-terminal amino acid via an engineered cysteine protease domain.Communications biology · 2025Article
- Tailored endothelialization enabled by engineered endothelial cell vesicles accelerates remodeling of small-diameter vascular grafts.Bioactive materials · 2024Article
- CaNature communications · 2024Article
- A "One-Step" Strategy for the Global Characterization of Core-Fucosylated Glycoproteome.JACS Au · 2024Article
- Cut-insert-stitch editing reaction (CIStER) sequence for surgical chemical glycan editing.Communications chemistry · 2024Article
- In Situ Glycan Analysis and Editing in Living Systems.JACS Au · 2024Review
- Mass Spectrometry Strategies forCells · 2024Review
- Synthetic Catalysts for Selective Glycan Cleavage from Glycoproteins and Cells.Journal of the American Chemical Society · 2024Article
- Glycan Complexity and Heterogeneity of Glycoproteins in Somatic Extracts and Secretome of the Infective Stage of the Helminth Fasciola hepatica.Molecular & cellular proteomics : MCP · 2023Article
- Divergent Enzymatic Assembly of a Comprehensive 64-Membered IgG N-Glycan Library for Functional Glycomics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Glycosylation in SARS-CoV-2 variants: A path to infection and recovery.Biochemical pharmacology · 2022Review
- N-Linked Glycosylation in Chinese Hamster Ovary Cells Is Critical for Insulin-like Growth Factor 1 Signaling.International journal of molecular sciences · 2022Article
- Carbohydrate-active enzymes (CAZymes) in the gut microbiome.Nature reviews. Microbiology · 2022Review
- Seeing the forest through the trees: characterizing the glycoproteome.Trends in biochemical sciences · 2022Review
- Sweet impersonators: Molecular mimicry of host glycans by bacteria.Glycobiology · 2022Review
- Chemical reporters to study mammalian O-glycosylation.Biochemical Society transactions · 2021Review
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Authors and funding
16 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell surfaces are glycosylated in various ways with high heterogeneity, which usually leads to ambiguous conclusions about glycan-involved biological functions. Here, we describe a two-step chemoenzymatic approach for N-glycan-subtype-selective editing on the surface of living cells that consists of a first 'delete' step to remove heterogeneous N-glycoforms of a certain subclass and a second 'insert' step to assemble a well-defined N-glycan back onto the pretreated glyco-sites. Such glyco-edited cells, carrying more homogeneous oligosaccharide structures, could enable precise understanding of carbohydrate-mediated functions. In particular, N-glycan-subtype-selective remodeling and imaging with different monosaccharide motifs at the non-reducing end were successfully achieved. Using a combination of the expression system of the Lec4 CHO cell line and this two-step glycan-editing approach, opioid receptor delta 1 (OPRD1) was investigated to correlate its glycostructures with the biological functions of receptor dimerization, agonist-induced signaling and internalization.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.