ArticleNature methods2020
Real-time 3D movement correction for two-photon imaging in behaving animals.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
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Who cites it
54 citing papers in PubMed, 85 citations in OpenAlex.
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- Technical development of two-photon optogenetic stimulation and its potential application to brain-machine interfaces.Neurophotonics · 2026Review
- High-throughput two-photon volumetric brain imaging in freely moving mice.Nature communications · 2025Article
- Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice.Nature communications · 2025Article
- Transport in the brain studied withNeurophotonics · 2025Review
- Suite3D: Volumetric cell detection for two-photon microscopy.bioRxiv : the preprint server for biology · 2025Article
- Moculus: an immersive virtual reality system for mice incorporating stereo vision.Nature methods · 2025Article
- Window into the Brain: In Vivo Multiphoton Imaging.ACS photonics · 2025Review
- Super-resolution imaging of fast morphological dynamics of neurons in behaving animals.Nature methods · 2025Article
- Multimodal Imaging Unveils the Impact of Nanotopography on Cellular Metabolic Activities.Chemical & biomedical imaging · 2024Article
- CRASH2p: Closed-loop Two Photon Imaging in a Freely Moving Animal.bioRxiv : the preprint server for biology · 2024Article
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- Neurophotonics beyond the surface: unmasking the brain's complexity exploiting optical scattering.Neurophotonics · 2024Article
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- Closed-loop experiments and brain machine interfaces with multiphoton microscopy.Neurophotonics · 2024Review
- Acousto-optic holography for pseudo-two-dimensional dynamic light patterning.APL photonics · 2024Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 3 countries.
Funding
Abstract
Two-photon microscopy is widely used to investigate brain function across multiple spatial scales. However, measurements of neural activity are compromised by brain movement in behaving animals. Brain motion-induced artifacts are typically corrected using post hoc processing of two-dimensional images, but this approach is slow and does not correct for axial movements. Moreover, the deleterious effects of brain movement on high-speed imaging of small regions of interest and photostimulation cannot be corrected post hoc. To address this problem, we combined random-access three-dimensional (3D) laser scanning using an acousto-optic lens and rapid closed-loop field programmable gate array processing to track 3D brain movement and correct motion artifacts in real time at up to 1 kHz. Our recordings from synapses, dendrites and large neuronal populations in behaving mice and zebrafish demonstrate real-time movement-corrected 3D two-photon imaging with submicrometer precision.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.