ArticleScientific reports2020
Bacteriophage K1F targets Escherichia coli K1 in cerebral endothelial cells and influences the barrier function.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Phage-steering permits antibody-mediated clearance of E. coli K1 from the gut.Nature communications · 2026Article
- Transcriptomic analysis of phage-mammalian cell interaction reveals diverse phage immunobarcodes.iScience · 2025Article
- Human bladder organoids model urinary tract infection and bacteriophage therapy.bioRxiv : the preprint server for biology · 2025Article
- Review
- Phage-Microbiota Crosstalk: Implications for Central Nervous System Disorders.International journal of molecular sciences · 2025Review
- Temporal transcriptomics uncover dynamic interactions between pathogenicFrontiers in microbiology · 2025Article
- E. coli Meningitis.Methods in molecular biology (Clifton, N.J.) · 2025Review
- The Discovery of Phages in the Substantia Nigra and Its Implication for Parkinson's Disease.Research (Washington, D.C.) · 2025Article
- Evaluation of effectiveness of bacteriophage purification methods.Virology journal · 2024Article
- Review
- Liver sinusoidal cells eliminate blood-borne phage K1F.mSphere · 2024Article
- IRAK3 Knockout and Wildtype THP-1 Monocytes as Models for Endotoxin Detection Assays andInternational journal of molecular sciences · 2023Article
- Mammalian cells internalize bacteriophages and use them as a resource to enhance cellular growth and survival.PLoS biology · 2023Article
- Bacteriophages as Potential Clinical Immune Modulators.Microorganisms · 2023Review
- Genetic Engineering of Bacteriophage K1F with Human Epidermal Growth Factor to Enhance Killing of IntracellularACS synthetic biology · 2023Article
- Review
- A bioorthogonal chemistry approach to detect the K1 polysialic acid capsule inRSC chemical biology · 2023Article
- Investigating the effect of bacteriophages on bacterial FtsZ localisation.Frontiers in cellular and infection microbiology · 2022Article
- Polymer-Mediated Cryopreservation of Bacteriophages.Biomacromolecules · 2021Article
- Novel Host Recognition Mechanism of the K1 Capsule-Specific Phage ofJournal of virology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Bacterial neonatal meningitis results in high mortality and morbidity rates for those affected. Although improvements in diagnosis and treatment have led to a decline in mortality rates, morbidity rates have remained relatively unchanged. Bacterial resistance to antibiotics in this clinical setting further underlines the need for developing other technologies, such as phage therapy. We exploited an in vitro phage therapy model for studying bacterial neonatal meningitis based on Escherichia coli (E. coli) EV36, bacteriophage (phage) K1F and human cerebral microvascular endothelial cells (hCMECs). We show that phage K1F is phagocytosed and degraded by constitutive- and PAMP-dependent LC3-assisted phagocytosis and does not induce expression of inflammatory cytokines TNFα, IL-6, IL-8 or IFNβ. Additionally, we observed that phage K1F temporarily decreases the barrier resistance of hCMEC cultures, a property that influences the barrier permeability, which could facilitate the transition of immune cells across the endothelial vessel in vivo. Collectively, we demonstrate that phage K1F can infect intracellular E. coli EV36 within hCMECs without themselves eliciting an inflammatory or defensive response. This study illustrates the potential of phage therapy targeting infections such as bacterial neonatal meningitis and is an important step for the continued development of phage therapy targeting antibiotic-resistant bacterial infections generally.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.