ArticleScientific reports2020
Green analytical method for the simultaneous analysis of cytochrome P450 probe substrates by poly(N-isopropylacrylamide)-based temperature-responsive chromatography.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 27 citations in OpenAlex.
- Temperature-modulated separation of therapeutic cells, viral vectors, and exosomes using functional polymers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- Article
- Harvesting methods of umbilical cord-derived mesenchymal stem cells from culture modulate cell properties and functions.Regenerative therapy · 2024Article
- Bioanalytical technologies using temperature-responsive polymers.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Review
- Thermoresponsive bio-affinity interfaces for temperature-modulated selective capture and release of targeted exosomes.Materials today. Bio · 2023Article
- Chromatography columns packed with thermoresponsive-cationic-polymer-modified beads for therapeutic drug monitoring.Scientific reports · 2022Article
- Temperature-responsive mixed-mode column for the modulation of multiple interactions.Scientific reports · 2022Article
- Temperature responsive chromatography for therapeutic drug monitoring with an aqueous mobile phase.Scientific reports · 2021Article
- Effect of pore diameter on the elution behavior of analytes from thermoresponsive polymer grafted beads packed columns.Scientific reports · 2021Article
- Effective Separation for New Therapeutic Modalities Utilizing Temperature-responsive Chromatography.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
High-performance liquid chromatography (HPLC) is the most common analytical method practiced in various fields and used for analysis of almost all drug compounds in the pharmaceutical industries. During drug development, an evaluation of potential drug interaction with cytochrome P450 (CYP) is essential. A "cocktail" approach is often used in drug development to evaluate the effect of a drug candidate on multiple CYP enzymes in a single experiment. So far, simultaneous analysis of multiple CYP substrates, which have greatly different structure and physicochemical properties, has required organic solvents and mobile phase gradient methods. However, despite the recent emphasis on environmental protection, analytical methods that use only aqueous solvents without the use of organic solvents for separation have not been studied well. This study sought to develop the simultaneous analysis of multiple CYP substrates by using poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive chromatography with only aqueous solvents and isocratic methods. Good separation of multiple CYP substrates was achieved without using organic solvents and any gradient methods by temperature-responsive chromatography utilizing a P(NIPAAm-co-n-butyl methacrylate (BMA))- and P(NIPAAm-co-N-acryloyl L-tryptophan methyl ester (L-Trp-OMe))-grafted silica column. Overall, PNIPAAm-based temperature-responsive chromatography represents a remarkably simple, versatile, and environmentally friendly bioanalytical method for CYP substrates and their metabolites.
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