ArticleMicrobiome2020
Freshwater viral metagenome reveals novel and functional phage-borne antibiotic resistance genes.
Article in Microbiome, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 214 citations in OpenAlex.
- Comparative analysis of gut viromes in four penguin species reveals diverse novel viruses and host-associated differences.mSphere · 2026Article
- A diverse gut virome in natural populations of Drosophila melanogaster.Animal microbiome · 2026Article
- On the Low Abundance of Antibiotic Resistance Genes in Bacteriophage Genomes and Their Random Acquisition via Specialized Transduction.Genome biology and evolution · 2026Article
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- Phages drive the dissemination of antibiotic resistance genes by facilitating host adaptation to heavy metal stress.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mining of food metagenomes reveals an unexplored diversity of dsDNA bacteriophages.NPJ biofilms and microbiomes · 2026Article
- Diversity and ecological potential of sediment viruses from Chinese continental shelf seas.NPJ biofilms and microbiomes · 2026Article
- Mapping 25 years of research on gut microbiota and antibiotic resistance: bibliometric insights and future directions.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Review
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- Age-dependent patterns of the gut microbiome, antibiotic resistome, and pathogenicity in captive koalas (Phascolarctos cinereus).Communications biology · 2025Article
- Metagenomic analysis of bacteriome and phageome of wastewater from a ceramic factory in Türkiye.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Mapping the underlying drivers of resistome risk across diverse environments.Research square · 2025Article
- Eco-evolutionary strategies drive viral diversification in nutrient-poor soils across elevation gradients.National science review · 2025Article
- Evolutionary selection of trimethoprim-resistantScience advances · 2025Article
- Global biogeography of airborne viruses in public transit systems and their host interactions.Microbiome · 2025Article
- Unique plastisphere viromes with habitat-dependent potential for modulating global methane cycle.Nature communications · 2025Article
- Metagenomic analysis reveals how multiple stressors disrupt virus-host interactions in multi-trophic freshwater mesocosms.Nature communications · 2025Article
- Two novel trimethoprim resistance genes,Antimicrobial agents and chemotherapy · 2025Article
- Review of the Presence and Phage-Mediated Transfer of ARGs in Biofilms.Microorganisms · 2025Review
- Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2025Review
27 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundAntibiotic resistance developed by bacteria is a significant threat to global health. Antibiotic resistance genes (ARGs) spread across different bacterial populations through multiple dissemination routes, including horizontal gene transfer mediated by bacteriophages. ARGs carried by bacteriophages are considered especially threatening due to their prolonged persistence in the environment, fast replication rates, and ability to infect diverse bacterial hosts. Several studies employing qPCR and viral metagenomics have shown that viral fraction and viral sequence reads in clinical and environmental samples carry many ARGs. However, only a few ARGs have been found in viral contigs assembled from metagenome reads, with most of these genes lacking effective antibiotic resistance phenotypes. Owing to the wide application of viral metagenomics, nevertheless, different classes of ARGs are being continuously found in viral metagenomes acquired from diverse environments. As such, the presence and functionality of ARGs encoded by bacteriophages remain up for debate.
resultsWe evaluated ARGs excavated from viral contigs recovered from urban surface water viral metagenome data. In virome reads and contigs, diverse ARGs, including polymyxin resistance genes, multidrug efflux proteins, and β-lactamases, were identified. In particular, when a lenient threshold of e value of ≤ 1 × e
conclusionOur results showed that viruses in the environment carry as-yet-unreported functional ARGs, albeit in small quantities. We thereby suggest that environmental bacteriophages could be reservoirs of widely variable, unknown ARGs that could be disseminated via virus-host interactions. Video abstract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.