ArticleBiomaterials2020
Tunable bioactivity and mechanics of collagen-based tissue engineering constructs: A comparison of EDC-NHS, genipin and TG2 crosslinkers.
Article in Biomaterials, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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Who cites it
45 citing papers in PubMed, 147 citations in OpenAlex.
- Collagen-Based Microspheres for Biomedical Applications in Drug Delivery and Tissue Engineering.Biomimetics (Basel, Switzerland) · 2026Review
- Comparative Analysis of Genipin-Crosslinked Carp Collagen Gels and Their Effects on L929 Fibroblast Growth and Differentiation.Gels (Basel, Switzerland) · 2026Article
- Unraveling the Potential ofBiomacromolecules · 2026Article
- Catalyst-Free Collagen Filament Crosslinking for Engineering Anisotropic and Mechanically Robust Tissue Scaffolds.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- PEGylated PLGA Nanoformulations For Effective Immunomodulatory Actors In Non-Small Cell Lung Cancer.International journal of nanomedicine · 2026Review
- A photo-crosslinkable hyaluronic acid-collagen hydrogel maintains epithelial integrity and supports intestinal organoid stemness.Regenerative biomaterials · 2026Article
- Chemical Crosslinking of Acid Soluble Collagen Fibres.Biomimetics (Basel, Switzerland) · 2025Review
- Collagen-elastin dermal scaffolds enhance tissue regeneration and reduce scarring in preclinical models.Materials today. Bio · 2025Article
- Exo-hydrogel therapy: a revolutionary approach to managing diabetic complications.Journal of nanobiotechnology · 2025Review
- Poloxamer-based hydrogel with EGCG and rhEGF for diabetic foot ulcer treatment.Journal of materials science. Materials in medicine · 2025Article
- PEGylated PLGA nanoparticles: unlocking advanced strategies for cancer therapy.Molecular cancer · 2025Review
- Physicochemical Reinforcement of Hydrophilic Gelatin Films by Fibroin Nanofibrils Prepared via Cellulose-Assisted Self-Assembly.Biomacromolecules · 2025Article
- Article
- Advances and challenges in decellularized adipose tissue based composite hydrogels for adipose tissue regeneration: a review over the last fifteen years.Theranostics · 2025Review
- Porous PLLA microspheres dispersed in HA/collagen hydrogel as injectable facial fillers to enhance aesthetic effects.Regenerative biomaterials · 2025Article
- Current Status of Bioprinting Using Polymer Hydrogels for the Production of Vascular Grafts.Gels (Basel, Switzerland) · 2024Review
- Construction of living-cell tissue engineered amniotic membrane for ocular surface disease.BMC ophthalmology · 2024Article
- The Influence of Various Crosslinking Conditions of EDC/NHS on the Properties of Fish Collagen Film.Marine drugs · 2024Article
- Preparation and Modification of Collagen/Sodium Alginate-Based Biomedical Materials and Their Characteristics.Polymers · 2024Article
- Trophoblast-Targeted Liposomes for Placenta-Specific Drug Delivery.Methods in molecular biology (Clifton, N.J.) · 2024Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to its ubiquity and versatility in the human body, collagen is an ideal base material for tissue-engineering constructs. Chemical crosslinking treatments allow precise control of the biochemical and mechanical properties through macromolecular modifications to the structure of collagen. In this work, three key facets regarding the collagen crosslinking process are explored. Firstly, a comparison is drawn between the carbodiimide-succinimide (EDC-NHS) system and two emerging crosslinkers utilising alternate chemistries: genipin and tissue transglutaminase (TG2). By characterising the chemical changes upon treatment, the effect of EDC-NHS, genipin and TG2 crosslinking mechanisms on the chemical structure of collagen, and thus the mechanical properties conferred to the substrate is explored. Secondly, the relative importance of mechanical and biochemical cues on cellular phenomena are investigated, including cell viability, integrin-specific attachment, spreading and proliferation. Here, we observe that for human dermal fibroblasts, long-term, stable proliferation is preconditioned by the availability of suitable binding sites, irrespective of the substrate modulus post-crosslinking. Finally, as seen in the graphical abstract we show that by choosing the appropriate crosslinker chemistries, a materials selection map can be drawn for collagen films, encompassing both a range of tensile modulus and fibroblast proliferation which can be modified independently. Thus, in addition to a range of parameters that can be modified in collagen constructs, we demonstrate a route to obtaining tunable bioactivity and mechanics in collagen constructs is uncovered, that is exclusively driven by the crosslinking process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.